Neural Stem Cell Assays (eBook)

Navjot Kaur, Mohan C. Vemuri (Herausgeber)

eBook Download: PDF

2014 | 1. Auflage
288 Seiten
John Wiley & Sons (Verlag)
978-1-118-30827-1 (ISBN)

Infos

Neural stem cells offer a valuable model system for delineating the cellular and developmental processes in normal and diseased states of the central nervous system. In particular, neural stem cells have huge potential in regenerative medicine, owing to their expansion capability in culture and the ability to differentiate into multiple sub-neural lineages.

Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. Including an overview of progress in the field over the past decade, Neural Stem Cell Assays is a one-stop reference for consistent methods and reliable tools that span the entire assay work flow, from isolation or generation of neural stem cells to characterization, manipulation and final application of neural stem cells in disease paradigms such as Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis.

An excellent source of information for academic, pharmaceutical and biotechnology researchers who are new to the neural stem cell field, Neural Stem Cell Assays is an invaluable to experienced users who wish to integrate newly developed tools and technologies into their workflow. The book also covers important course material for students at the undergraduate and graduate level who are learning the basics of neural stem cell cultures, and differentiation to sub-neural lineages.

Autor

Navjot Kaur, Ph.D, is Staff Scientist in the Life Technologies division of the Invitrogen Corporation. His research experience covers a wide range of cell biology, protein biochemistry and molecular biology concepts, with special expertise in cell culture and cryopreservation media development, cell-based assays, primary cells, and stable cell lines. Mohan C. Vemuri, Ph.D.,MBA is director of stem cell research and development in the Life Technologies division of Invitrogen & Applied Biosystems. The author of designs of peer-reviewed articles in the field, Dr. Vemuri is also a co-editor of three books on stem cells and stem cell cultures.

Inhalt

1. Neural Differentiation of Pluripotent Stem Cells
Mohan C. Vemuri

2. The History and Design of Assays for the Identification, Characterization of Neural Stem Cells
Sharon A. Louis and Brent A. Reynolds

3. Culture of Human Neural Stem Cells
Soojung Shin, Yiping Yan, and Mohan C. Vemuri

4. GMP-compatible Production and Expansion of Human Neural Stem Cells
David J. Brick, Alexander E. Stover, Hubert E. Nethercott, Maria G. Banuelos, and Philip H. Schwartz

5. Primary Rat Neural Cultures
Navjot Kaur

6. Cryopreservation of Human Neural Stem and Progenitor Cells
Profico D., Sgaravizzi G., Projetti Pensi M., Muzi G., Ricciolini C., Gelati M., and Vescovi A.L

7. Cryopreservation and Recovery of Primary Rat Neural Cells
Rhonda Newman and Navjot Kaur

8. Efficient Induction and Scale up Generation of Neural Stem Cells from Human Pluripotent Stem Cells
Yiping Yan, Soojung Shin, and Mohan C. Vemuri

9. Directed differentiation of Human NSC/NPC into Dopaminergic Neurons
Andrzej Swistowski and Xianmin Zeng

10. In vitro Differentiation of Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain Dopaminergic Neurons
Brigham J Hartley, Bradley Watmuff, Cameron PJ Hunt, John M Haynes, and Colin W Pouton

11. Generation of Astrocytes from Human Pluripotent Stem Cells using a Defined System
Atossa Shaltouki and Xianmin Zeng

12. Directed differentiation of human PSC into Oligodendrocytes
Anu Hyysalo and Susanna Narkilahti

13. Directed differentiation towards neural retinal cells
Deepak A. Lamba

14. Directed differentiation of Photoreceptors and Retinal Pigment Epithelium from adult Mouse Retinal Stem Cells
Brian G. Ballios, Valeria Marigo, and Derek van der Kooy

15. Induction of Schwann cells from bone marrow mesenchymal stem cells
Shohei Wakao, Masaaki Kitada, and Mari Dezawa

16. Neural Cell Viability Assays
Bonnie J. Hanson and Michael K. Hancock

17. Genetic modification of pluripotent stem cell derived human neural progenitor cells
Jamie M. Chilton and Steven L. Stice

18. Electrophysiology of Neurons
Christine M. Wroge and Steven Mennerick

19. Induction and transplantation of Neural Stem Cells from Human Pluripotent Stem Cells in Experimental Models (rat) of Parkinson's Disease
Makoto Motono, Asuka Morizane, and Jun Takahashi

20. Transplantation of neural stem cells and progenitors in animal models of disease
Joseph F. Bonner and Itzhak Fischer

21. Morphometric Assays for Neural Differentiation and Synaptic Development of Human iPSC-Derived NSC
Barbara Calabrese, Leif Dehmelt, and Shelley Halpain

22. BacMam Transduction of Neural Stem Cells
Rene Quintanilla and Uma Lakshmipathy

23. The Proximity Ligation Assay: A High Throughput Technique for Protein Analysis in Neuroscience
Michela Zaltieri, PierFranco Spano, Cristina Missale, and Arianna Bellucci

24. Quantitation of micro-RNA expression in neural stem cells using TaqMan assays
Xavier Nissan and Alexandra Benchoua

25. Genetic reporter cell lines: Tools for stem cell biology and drug discovery
Cameron PJ Hunt, Bradley Watmuff, Brigham J Hartley, Colin W Pouton, and John M Haynes

Produkt-Details

• Erscheint lt. Verlag: 24.11.2014
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Neurologie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Technik
• Schlagworte: Biopharmaceuticals & Pharmaceutical Biotechnology • Biopharmazeutika u. Pharmazeutische Biotechnologie • Biowissenschaften • Cell Biology • Chemie • Chemistry • Life Sciences • Neuroscience • Neurowissenschaften • Stammzelle • Zellbiologie
• ISBN-10: 1-118-30827-1 / 1118308271
• ISBN-13: 978-1-118-30827-1 / 9781118308271