Essential Molecular Biology
Seiten
2001
|
2nd Revised edition
Oxford University Press (Verlag)
978-0-19-963645-7 (ISBN)
Oxford University Press (Verlag)
978-0-19-963645-7 (ISBN)
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In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications
to suit personal requirements.
Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications
to suit personal requirements.
Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.
1. Strategies for research in molecular biology ; 2. Construction of genomic libraries in lambda and cosmic vectors ; 3. Construction of a cDNA library ; 4. Nucleic acid labelling and detection ; 5. Immobilization of nucleic acids and hybridization analysis ; 6. DNA sequencing ; 7. The polymerase chain reaction ; 8. Protein expression in Escherichia coli ; 9. Transcript mapping ; APPENDICES ; 1. Health hazards and safety procedures ; 2. Equipment for molecular biology research ; 3. Important Escherichia coli strains ; 4. Recipes and general procedures ; 5. Restriction endonucleases ; 6. DNA and RNA modification enzymes ; 7. List of suppliers' addresses
| Reihe/Serie | Practical Approach Series ; 255 |
|---|---|
| Zusatzinfo | numerous line figures, 9 halftones |
| Verlagsort | Oxford |
| Sprache | englisch |
| Maße | 194 x 253 mm |
| Gewicht | 792 g |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Technik ► Lebensmitteltechnologie | |
| ISBN-10 | 0-19-963645-1 / 0199636451 |
| ISBN-13 | 978-0-19-963645-7 / 9780199636457 |
| Zustand | Neuware |
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