Molecular Biology Problem Solver (eBook)

A Laboratory Guide

Alan S. Gerstein (Autor)

eBook Download: PDF

2004 | 1. Auflage
596 Seiten
John Wiley & Sons (Verlag)
978-0-471-46103-6 (ISBN)

Infos

Most research in the life sciences involves a core set of
molecular-based equipment and methods, for which there is no
shortage of step-by-step protocols. Nonetheless, there remains an
exceedingly high number of inquiries placed to commercial technical
support groups, especially regarding problems.

Molecular Biology Problem Solver: A Laboratory
Guide asks the reader to consider crucial questions, such
as:

* Have you selected the most appropriate research strategy?

* Have you identified the issues critical to your successful
application of a technique?

* Are you familiar with the limitations of a given
technique?

* When should common procedural rules of thumb not be
applied?

* What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and
laboratory practices, with the aim of offering a firm understanding
of how techniques and procedures work, as well as how to avoid
problems. Some major issues explored by the book's expert
contributors include:

* Working safely with biological samples and radioactive
materials

* DNA and RNA purification

* PCR

* Protein and nucleid acid hybridization

* Prokaryotic and eukaryotic expression systems

* Properly using and maintaining laboratory equipment

Autor

ALAN GERSTEIN and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.

Inhalt

Preface.

Contributors.

Preparing for Success in the Laboratory (P. Franciskovich).

Getting What You Need from a Supplier (T. Tyre & G.
Krueger).

The Preparation of Buffers and Other Solutions: A Chemist's
Perspective (E. Pfannkoch).

How to Properly Use and Maintain Laboratory Equipment (T. Troutman,
et al.).

Working Safely with Biological Samples (C. Haidaris & E.
Brownlow).

Working Safely with Radioactive Materials (W. Volny).

DNA Purification (S. Herzer).

RNA Purification (L. Martin, et al.).

Restriction Endonucleases (D. Robinson, et al.).

Nucleotides, Oligonucleotides, and Polynucleotides (A.
Gerstein).

PCR (K. Aoyagi).

Electrophoresis (M. Booz).

Western Blotting (P. Riis).

Nucleic Acid Hybridization (S. Herzer & D. Englert).

E. coli Expression Systems (P. Bell).

Eukaryotic Expression (J. Trill, et al.).

Index.

Rezensionen

"It will strengthen your ability to prevent, identify and solve problems in the lab..." (Medical Laboratory World, October 2001)

Produkt-Details

• Erscheint lt. Verlag: 7.4.2004
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Technik
• Schlagworte: Biologie / Grundlagen • Biowissenschaften • Cell & Molecular Biology • Life Sciences • Zell- u. Molekularbiologie
• ISBN-10: 0-471-46103-2 / 0471461032
• ISBN-13: 978-0-471-46103-6 / 9780471461036