Molecular Biology Problem Solver
Wiley-Liss Inc.,U.S. (Verlag)
978-0-471-37972-0 (ISBN)
Have you selected the most appropriate research strategy?
Have you identified the issues critical to your successful application of a technique?
Are you familiar with the limitations of a given technique?
When should common procedural rules of thumb not be applied?
What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
Working safely with biological samples and radioactive materials
DNA and RNA purification
PCR
Protein and nucleid acid hybridization
Prokaryotic and eukaryotic expression systems
Properly using and maintaining laboratory equipment
ALAN GERSTEIN and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.
Preface.
Contributors.
Preparing for Success in the Laboratory (P. Franciskovich).
Getting What You Need from a Supplier (T. Tyre & G. Krueger).
The Preparation of Buffers and Other Solutions: A Chemist's Perspective (E. Pfannkoch).
How to Properly Use and Maintain Laboratory Equipment (T. Troutman, et al.).
Working Safely with Biological Samples (C. Haidaris & E. Brownlow).
Working Safely with Radioactive Materials (W. Volny).
DNA Purification (S. Herzer).
RNA Purification (L. Martin, et al.).
Restriction Endonucleases (D. Robinson, et al.).
Nucleotides, Oligonucleotides, and Polynucleotides (A. Gerstein).
PCR (K. Aoyagi).
Electrophoresis (M. Booz).
Western Blotting (P. Riis).
Nucleic Acid Hybridization (S. Herzer & D. Englert).
E. coli Expression Systems (P. Bell).
Eukaryotic Expression (J. Trill, et al.).
Index.
| Erscheint lt. Verlag | 12.10.2001 |
|---|---|
| Verlagsort | New York |
| Sprache | englisch |
| Maße | 179 x 253 mm |
| Gewicht | 1001 g |
| Einbandart | Paperback |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| ISBN-10 | 0-471-37972-7 / 0471379727 |
| ISBN-13 | 978-0-471-37972-0 / 9780471379720 |
| Zustand | Neuware |
| Haben Sie eine Frage zum Produkt? |
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