Medical BioMethods Handbook (eBook)

John M. Walker, Ralph Rapley (Herausgeber)

eBook Download: PDF

2007 | 2005
XVI, 644 Seiten
Humana Press (Verlag)
978-1-59259-870-0 (ISBN)

Infos

John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.

Inhalt

Basic Techniques in Molecular Biology
Ralph Rapley

Probe Design, Production, and Applications
Marilena Aquino de Muro

Restriction Enzymes: Tools in Clinical Research
Gareth J. S. Jenkins

Southern Blotting as a Diagnostic Method
Bronwen Harvey and Pirkko Soundy

Western Blotting as a Diagnostic Method
Pirkko Soundy and Bronwen Harvey

Principles and Medical Applications of the Polymerase Chain Reaction
Bimal D. M. Theophilus

Single-Strand Conformation Polymorphism (SSCP) Analysis
Igor Vorechovsky

Denaturing Gradient Gel Electrophoresis (DGGE)
Jeroen H. Roelfsema and Dorien J. M. Peters

Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Sequencing
Anu Suomalainen and Ann-Christine Syvänen

Introduction to Capillary Electrophoresis of DNA: Biomedical Applications
Beatriz Sanchez-Vega

Mapping Techniques
Simon G. Gregory

Dideoxyfingerprinting for Mutation Detection
Ioannis Bossis, Antonios Voutetakis, and Constantine A. Stratakis

Conformation-Sensitive Gel Electrophoresis
Marian Hill

Amplification Refractory Mutation System and Molecular Diagnostics
Richard Kitching and Arun Seth

Ligase Chain Reaction
Carla Osiowy

Chemical Cleavage of Mismatch: Theory and Clinical Applications
Neil V. Whittock and Louise Izatt

The Protein Truncation Test
Johan T. den Dunnen

Linkage, Allele Sharing, and Association
Mara Giordano

Single-Nucleotide Polymorphisms: Technology and Applications
Cyril Mamotte, Frank Christiansen, and Lyle J. Palmer

cDNA Microarrays
Phillip G. Febbo

Nucleic Acid Sequence-Based Amplification
Katherine Loens, D. Ursi, H. Goosens, and M. Ieven

The Oligonucleotide Ligation Assay
Faye A. Eggerding

QuantitativeTaqMan Real-Time PCR: Diagnostic and Scientific Applications
Jörg Dötsch, Ellen Schoof, and Wolfgang Rascher

Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine
Elizabeth L. Rugg and Gareth J. Magee

Quantitative PCR
David Sugden

Liquid Chromatography-Mass Spectrometry of Nucleic Acids
Herbert Oberacher and Walther Parson

Comparative Genomic Hybridization in Clinical and Medical Research
Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin

Bioinformatics Tools for Gene and Protein Sequence Analysis
Bernd H. A. Rehm and Frank Reinecke

In Situ Hybridization
Amanda D. Watters

Enzyme-Linked Immunosorbent Assay
William J. Jordan

Protein Therapeutics: Mouse, Humanized, and Human Antibodies
Benny K. C. Lo

Karyotyping
Avery A. Sandberg, John F. Stone, and Zhong Chen

Microsatellite Analysis
Rachel E. Ibbotson and Anton E. Parker

Analysis of Chromosomal Translocations
Andreas Hochhaus

Differential Display: Theory and Applications
Irina Gromova, Pavel Gromov, and Julio E. Celis

Techniques for Gene Expression Profiling
Mark P. Richards

Capillary Electrophoresis in Clinical Analysis
Margaret A. Jenkins and Sujiva Ratnaike

Flow Cytometry in the Biomedical Arena
James L. Weaver and Maryalice Stetler-Stevenson

Immunocytochemistry
Patricia A. Fetsch

Ribotyping in Clinical Microbiology
Patricia Severino and Sylvain Brisse

Diagnostic Applications of Protein Microarrays
Samir Hanash

The Human Genome Project
Rahul Chodhari and Eddie Chung

Prenatal Diagnosis of Inborn Errors of Metabolism
Guy T. N. Besley

Gene Therapy: Methods and Application
Stella B. Somiari

Index

Produkt-Details

• Erscheint lt. Verlag: 9.10.2007
• Reihe/Serie: Springer Protocols Handbooks
• Reihe/Serie: Springer Protocols Handbooks
• Zusatzinfo: XVI, 644 p.
• Verlagsort: Totowa
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Gesundheitsfachberufe
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Technik
• Schlagworte: Allele • Bioinformatics • DNA • gene expression • genes • Hybridization • Ligation • microarray • Molecular Biology • PCR • Quantitative PCR • Southern Blot • Western blotting
• ISBN-10: 1-59259-870-6 / 1592598706
• ISBN-13: 978-1-59259-870-0 / 9781592598700