MicroRNA Interference Technologies (eBook)

Zhiguo Wang (Autor)

eBook Download: PDF

2009 | 2009
XII, 194 Seiten
Springer Berlin (Verlag)
978-3-642-00489-6 (ISBN)

Infos

MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of around 22-nucleotides long, have rapidly emerged as one of the key governors of the gene expression regulatory program in cells of varying species, with ever-increasing implications in the control of the fundamental biological processes and in the pathogenesis of adult humans. The exciting findings in this field have inspired us with a premise and a promise that miRNAs will ultimately be taken to the heart for therapy of human disease. While miRNAs have been considered potential therapeutic targets for disease treatment, it remains obscured what strategies we can use to achieve the goal. In the past years, we have witnessed a rapid evolving of many creative, innovative, inventive strategies and methodologies pertinent to miRNA research and applications. These technologies have convincingly demonstrated their efficacy and reliability in producing gain-of-function or loss-of-function of miRNAs through targeting miRNA expression/biogenesis/function, providing new tools for elucidating miRNA functions and opening up a new avenue for the development of new agents targeting miRNAs for therapeutic aims. The present book provides comprehensive descriptions of these technologies and their applications to miRNA research and to new drug design for miRNA-related diseases. It starts with an overview of up-to-date knowledge of miRNA biology and the potential of miRNAs as therapeutic targets for human disease, followed by an introduction of the new concept of miRNA interference (miRNAi) and the perspectives of miRNAi technologies in general terms. In the following, each chapter introduces one of the miRNAi technologies with detailed descriptions of state-of-the-art design, procedures, principles and applications to basic research, R and D and clinical therapy.

Inhalt

Preface 5
Acknowledgement 7
Contents 9
miRNAs Targeting and Targeting miRNAs 13
miRNA Interference Technologies: An Overview 70
Synthetic Canonical miRNA Technology 85
miRNA Mimic Technology 102
Multi-miRNA Hairpins and Multi-miRNA Mimics Technologies 110
miRNA Transgene Technology 120
Anti-miRNA Antisense Oligonucleotides Technology 136
Multiple-Target Anti-miRNA Antisense Oligonucleotides Technology 153
miRNA Sponge Technology 160
miRNA-Masking Antisense Oligonucleotides Technology 167
Sponge miR-Mask Technology 173
miRNA Knockout Technology 180
Dicer Inactivation Technology 188
Index 196

Produkt-Details

• Erscheint lt. Verlag: 11.6.2009
• Zusatzinfo: XII, 194 p. 23 illus., 17 illus. in color.
• Verlagsort: Berlin
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Technik
• Schlagworte: Activation • gene expression • gene therapy • interference technology • miRNA • miRNAs • Nucleotide
• ISBN-10: 3-642-00489-X / 364200489X
• ISBN-13: 978-3-642-00489-6 / 9783642004896