Mass Spectrometry-Based Proteomics

Buch | Hardcover

336 Seiten

2023 | 2023 ed.
Springer-Verlag New York Inc.
978-1-0716-3456-1 (ISBN)

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This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mass Spectrometry-Based Proteomics serves as an ideal guide to its subject for both novices in the field of proteomics as well as specialists.

lt;p>1. Increasing the Overall Proteome Coverage by Combining Protein Digestion by Tryp-N and Trypsin

Jade I. Hawksworth, Lode Denolf, Evy Timmerman, and Kris Gevaert

2. Cell Surface Biotinylation Using Furan Crosslinking Chemistry

Esperanza Fernandez, Laia Miret-Casals, Annemieke Madder, and Kris Gevaert

3. Studying Cellular Dynamics Using Proximity-Dependent Biotinylation: Somatic Cell Reprogramming

Reuben Samson, Francesco Zangari, and Anne-Claude Gingras

4. Virotrap: Trapping Protein Complexes in Virus-Like Particles

George D. Moschonas, Margaux De Meyer, Delphine De Sutter, Evy Timmerman, Petra Van Damme, and Sven Eyckerman

5. Thermal Proteome Profiling for Drug Target Identification and Probing of Protein States

Patricia Sauer and Marcus Bantscheff

6. Improved Coverage of the N-Terminome by Combining ChaFRADIC with Alternative Proteases

Xuehui Jiang, Ying Lao, Victor Spicer, and René P. Zahedi

7. Sensitive and High-Throughput Exploration of Protein N Termini by TMT-TAILS N-Terminomics

Konstantinos Kalogeropoulos, Louise Bundgaard, and Ulrich auf dem Keller

8. The Global Acetylation Profiling Pipeline for Quick Assessment of Protein N-Acetyltransferase Specificity In Cellulo

Thierry Meinnel, Jean-Baptiste Boyer, and Carmela Giglione

9. Systems-Wide Site-Specific Analysis of Glycoproteins

Kathirvel Alagesan and Emmanuelle Charpentier

10. Targeted Profiling of Protein Phosphorylation in Plants

Xiangyu Xu, Kris Gevaert, Ive De Smet, and Lam Dai Vu

11. Automated Sample Preparation for Mass Spectrometry-Based Clinical Proteomics

Torsten Müller, Mauro A. Cremonini, Georg Kliewer, and Jeroen Krijgsveld

12. Proteomics-Based Analysis and Diagnosis of Formalin-Fixed Paraffin-Embedded Amyloidosis Samples

Delphi Van Haver, Amélie Dendooven, and Francis Impens

13. A Robust and Clinically-Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics

Leyla A. Erozenci, Irene V. Bijnsdorp, Sander R. Piersma, and Connie R. Jimenez

14. Density-Based Fractionation of Cell Conditioned Medium to Prepare Proteomics Grade Extracellular Vesicles

Quentin Roux, Sarah Deville, and An Hendrix

15. Mass Spectrometry-Based Analysis of Histone Post-Translational Modifications from Laser Microdissected Samples

Roberta Noberini and Tiziana Bonaldi

16. Phosphoproteomics after Guanidinium Thiocyanate Extraction of Tissue Biopsies

Frank Rolfs, Richard R. de Goeij-de Haas, Jaco C. Knol, Sander R. Piersma, and Connie R. Jimenez

17. Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry

Zuzana Kalaninová, Lukás Fojtík, Josef Chmelík, Petr Novák, Michael Volný, and Petr Man

Erscheinungsdatum	06.09.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	57 Illustrations, color; 9 Illustrations, black and white; XI, 336 p. 66 illus., 57 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Chemie ► Analytische Chemie
Schlagworte	Cellular surfaceome • Clinical proteomics • Proteases • Protein modifications • proteome profiling
ISBN-10	1-0716-3456-9 / 1071634569
ISBN-13	978-1-0716-3456-1 / 9781071634561
Zustand	Neuware