Catharanthus roseus

Methods and Protocols

Vincent Courdavault, Sebastien Besseau (Herausgeber)

Buch | Softcover

319 Seiten

2023 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2351-0 (ISBN)

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lt;p>This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms

lt;p>1. RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms

Benoit St-Pierre, Samira Mahroug, Gregory Guirimand, Vincent Courdavault and Vincent Burlat

2. Imaging MS analysis in Catharanthus roseus

Kotaro Yamamoto, Katsutoshi Takahashi, Sarah E. O'Connor and Tetsuro Mimura

3. Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle

Chun-Han Su, Bo-Wei Wang, Ewelina P. Dutkiewicz, Cheng-Chih Hsu and Yu-Liang Yang

4. Sample preparation, data acquisition, and data analysis for ¹⁵N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus

Ryo Nakabayashi

5. Studying iridoid transport in Catharanthus roseus by grafting

Maisha Farzana, Mohammadamin Shahsavarani, Vincenzo De Luca and Yang Qu

6. Pictet-Spengler reaction for the chemical synthesis of strictosidine

Yingchao Dou, Laurent Evanno, Erwan Poupon and Guillaume Vincent

7. Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids

Pierre Le Pogam, Erwan Poupon, Pierre Champy and Mehdi A. Beniddir

8. Semi-Synthesis of Bis-Indole Alkaloid (-)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis

Alex Gardner and Rodrigo B. Andrade

9. RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus

Emily Amor Stander, Thomas Dugé de Bernonville, Vincent Courdavault

10. Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks

Thomas Dugé de Bernonville, Emily Amor Stander, Géraud Dugé de Bernonville, Sébastien Besseau and Vincent Courdavault

11. Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis

Tuan-Anh Minh Nguyen, Matthew McConnachie, Trinh-Don Nguyen and Thu-Thuy T. Dang

12. Ancestral sequence reconstruction for exploring alkaloid evolution

Benjamin R. Lichman

13. Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus

Mohammadamin Shahsavarani, Maisha Farzana, Vincenzo De Luca and Yang Qu

14. TARGETing transcriptional regulation in the medicinal plant Catharanthus roseus

Joana G. Guedes, Catarina Leitão, Catarina Meireles, Patrícia Duarte and Mariana Sottomayor

15. Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

Sanjay K. Singh, Barunava Patra, Joshua J. Singleton, Yongliang Liu,Priyanka Paul, Xueyi Sui, Nitima Suttipanta, Sitakanta Pattanaik and Ling Yuan

16. From Methylome to Integrative Analysis of Tissue-Specificity

Thomas Dugé de Bernonville, Christian Daviaud, Cristian Chaparro, Jörg Tost and Stéphane Maury

17. Tagging and capture of prenylated CaaX-proteins from plant cell cultures

Iliana Ribeiro, Eric Ducos, Nathalie Giglioli-Guivarc'h and Christelle Dutilleul

18. EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings

Samuel Mortensen, Lauren F. Cole, Diana Bernal-Franco, Suphinya Sathitloetsakun, Erin J. Cram and Carolyn W.T. Lee-Parsons

19. A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus

Konstantinos Koudounas, Ines Carqueijeiro, Pamela Lemos Cruz, Jennifer Perrin, Arnaud Lanoue, Audrey Oudin, Sébastien Besseau and Vincent Courdavault

20. Transient gene expression in Catharanthus roseus flower petals using agroinfiltration

Maite Colinas and Alain Goossens

21. De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants

Priyanka Verma, Shamshad Ahmad Khan and Ajay Kumar Mathur

22. Agrobacterium-mediated in planta transformation in Periwinkle

Dikki Pedenla Bomzan, Shilpashree H.B. and Dinesh A. Nagegowda

Erscheinungsdatum	28.06.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	59 Illustrations, color; 10 Illustrations, black and white; XIV, 319 p. 69 illus., 59 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	agrotransformation • EMS mutant library • Madagascar Periwinkle • Monoterpene Indole Alkaloids • protoplasting
ISBN-10	1-0716-2351-6 / 1071623516
ISBN-13	978-1-0716-2351-0 / 9781071623510
Zustand	Neuware