Catharanthus roseus
Springer-Verlag New York Inc.
978-1-0716-2348-0 (ISBN)
Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms
RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms.- Imaging MS analysis in Catharanthus roseus.- Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle.- Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus.- Studying iridoid transport in Catharanthus roseus by grafting.- Pictet-Spengler reaction for the chemical synthesis of strictosidine.- Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids.- Semi-Synthesis of Bis-Indole Alkaloid (–)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis.- RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus.- Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks.- Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis.- Ancestral sequence reconstruction for exploring alkaloid evolution.- Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus.- TARGETing transcriptional regulation in the medicinal plant Catharanthus roseus.- Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus.- From Methylome to Integrative Analysis of Tissue-Specificity.- Tagging and capture of prenylated CaaX-proteins from plant cell cultures.- EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings.- A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus.- Transient gene expression in Catharanthus roseus flower petals using agroinfiltration.- De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants.- Agrobacterium-mediated in planta transformation in Periwinkle.
Erscheinungsdatum | 27.06.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2505 |
Zusatzinfo | 59 Illustrations, color; 10 Illustrations, black and white; XIV, 319 p. 69 illus., 59 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | agrotransformation • EMS mutant library • Madagascar Periwinkle • Monoterpene Indole Alkaloids • protoplasting |
ISBN-10 | 1-0716-2348-6 / 1071623486 |
ISBN-13 | 978-1-0716-2348-0 / 9781071623480 |
Zustand | Neuware |
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