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Principles and Techniques of Biochemistry and Molecular Biology

Keith Wilson, John Walker (Herausgeber)

Buch | Hardcover
802 Seiten
2005 | 6th Revised edition
Cambridge University Press (Verlag)
978-0-521-82889-5 (ISBN)
CHF 119,95 inkl. MwSt
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This new and expanded edition of the bestselling textbook contains clear, concise explanations of a broad range of techniques encountered by students in practical classes and provides a comprehensive coverage of a wide range of bioscience methods, including cutting-edge techniques that have contributed to recent advances in biomedical research.
This is a new and expanded edition of the bestselling Principles and Techniques of Practical Biochemistry. It includes discussion of the theoretical principles, practical details and applications of the key experimental techniques that are routinely used in modern biochemistry and molecular biology, including those that have led to the emergence of the new disciplines of genomics, proteomics and bioinformatics. All chapters have been updated and new sections added to cover the principles of experimental design, the statistical analysis of quantitative analytical data, and the principles and practice of clinical biochemistry. here are new chapters covering cell culture, microscopy and mass spectrometry. To enhance student understanding of each topic, in-text worked examples are included in each chapter. The textbook will be essential reading for all bioscience students and pre-clinical medical students.

Keith Wilson is Professor Emeritus of Pharmacological Biochemistry and former Head of the Department of Biosciences, Dean of the Faculty of Natural Sciences, and Director of Research at the University of Hertfordshire. John Walker is Professor and Head of the School of Life Sciences and also Head of the Science Training Centre at the University of Hertfordshire.

Preface; List of contributors; List of abbreviations; 1. Basic priniciples Keith Wilson and John Fyffe; 2. Cell culture techniques Anwar R. Baydoun; 3. Centrifugation Kay Ohlendieck; 4. Microscopy Stephen W. Paddock; 5. Molecular biology, bioinformatics and basic techniques Ralph Rapley; 6. Recombinant DNA and genetic analysis Ralph Rapley; 7. Immunochemical techniques Robin Thorpe and Susan Thorpe; 8. Protein structure, purification, characterisation and functional analysis John Walker; 9. Mass spectrometric techniques Alistair Aitken; 10. Electrophoretic techniques John Walker; 11. Chromatographic techniques Keith Wilson; 12. Spectroscopic techniques I: atomic and molecular electronic spectroscopy Derek B. Gordon; 13. Spectroscopic techniques II: vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields; 14. Radioisotope techniques Robert J. Slater; 15. Enzymes Keith Wilson; 16. Cell membrane receptors and cell signalling Keith Wilson.

Erscheint lt. Verlag 21.3.2005
Zusatzinfo 66 Tables, unspecified; 5 Plates, color; 34 Halftones, unspecified; 287 Line drawings, unspecified
Verlagsort Cambridge
Sprache englisch
Maße 185 x 254 mm
Gewicht 1755 g
Themenwelt Naturwissenschaften Biologie Biochemie
ISBN-10 0-521-82889-9 / 0521828899
ISBN-13 978-0-521-82889-5 / 9780521828895
Zustand Neuware
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