Ion Mobility-Mass Spectrometry

Methods and Protocols

Giuseppe Paglia, Giuseppe Astarita (Herausgeber)

Buch | Softcover

312 Seiten

2020 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0032-0 (ISBN)

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This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as anideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules.- Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics.- Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry.- Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics.- The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines.- Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics.- Lipidomics by HILIC-Ion Mobility-Mass Spectrometry.- A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography–Ion Mobility-Mass Spectrometry.- Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS).- An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry.- Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics.- Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins.- Ion Mobility-Mass Spectrometry of Glycoconjugates.- Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry.- Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation.- Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility.- The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics.- MolFind2: A Protocol for Acquiring and Integrating MS3 Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics.- Collision Cross Section Calculations Using HPCCS.

Erscheinungsdatum	20.11.2020
Reihe/Serie	Methods in Molecular Biology ; 2084
Zusatzinfo	83 Illustrations, color; 19 Illustrations, black and white; XI, 312 p. 102 illus., 83 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Chemie ► Analytische Chemie
ISBN-10	1-0716-0032-X / 107160032X
ISBN-13	978-1-0716-0032-0 / 9781071600320
Zustand	Neuware