Neuroproteomics

Ka Wan Li (Herausgeber)

Buch | Softcover

218 Seiten

2020 | 2nd ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9664-3 (ISBN)

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This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.

Neuroproteomics: The Methods.- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue.- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice.- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue.- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics.- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3).- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip.- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes.- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry.- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons.- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures.- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data.- 2D-DIGE as a Tool in Neuroproteomics.- Neuropeptidomics of the Mammalian Brain.- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides.- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder.

Erscheinungsdatum	28.08.2020
Reihe/Serie	Neuromethods ; 146
Zusatzinfo	37 Illustrations, color; 18 Illustrations, black and white; XIII, 218 p. 55 illus., 37 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	DIGE • Laser Capture Microdissection • pluripotent brain stem cells • rodent brain regions • synaptic organelles
ISBN-10	1-4939-9664-9 / 1493996649
ISBN-13	978-1-4939-9664-3 / 9781493996643
Zustand	Neuware