The Nucleus

Buch | Hardcover

220 Seiten

2020 | 3rd ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0762-6 (ISBN)

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This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects.

Live-Cell Imaging and Analysis of Nuclear Body Mobility.- Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell.- Visualizing Chromatin Modifications in Isolated Nuclei.- Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture.- Studying Proton Gradients Across the Nuclear Envelope.- COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes.- Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq.- AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide.- Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID.- Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions.- Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres.- Macromolecular Crowding Measurementswith Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells.- Analysis of a Nuclear Intrinsically Disordered Proteome.- Timing of Cytosine Methylation on Newly-Synthesized RNA by Electron Microscopy.- The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants.

Erscheinungsdatum	31.07.2020
Reihe/Serie	Methods in Molecular Biology ; 2175
Zusatzinfo	30 Illustrations, color; 12 Illustrations, black and white; XI, 220 p. 42 illus., 30 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-0716-0762-6 / 1071607626
ISBN-13	978-1-0716-0762-6 / 9781071607626
Zustand	Neuware