Tissue Proteomics
Humana Press Inc. (Verlag)
978-1-4939-9304-8 (ISBN)
Authoritative and practical, Tissue Proteomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac Proteomics.- Discovery of Immune Reactive Human Proteins by High Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA.- HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells.- Characterization of Protein Complexes Using Chemical Cross-linking Coupled Electrospray Mass Spectrometry.- Optimization for Peptide Sample Preparation for Urine Peptidomics.- Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains.- Integrative Analysis of Proteomics Data to Obtain Clinically Relevant Markers.- Untargeted Screening of Urinary Peptides Using Off-line Nano-liquid Chromatography – MALDI-TOF/TOF Mass Spectrometry.- Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay.- In Situ Hybridization and Double Immunohistochemistry for the Detection of VEGF-A mRNA and CD34/Collagen IV Proteins in Renal Transplant Biopsies.- LC-SRM-based Targeted Quantification of Urinary Protein Biomarkers.- Fractionation of Soluble Proteins Using DEAE-, SP-, and Phenyl Sepharose Chromatographies for Proteomics.- GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue.- Targeted Proteomics Verification of Breast Cancer Biomarker Candidates.- Combination Strategy of Quantitative Proteomics Uncovers the Related-Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model.- Multiple Reaction Monitoring using Double Isotopologue Peptide Standards for Protein Quantification.- Isobaric Labeling Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome.- MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the same Formalin-Fixed, Paraffin-embedded Tissue Section.- Differential Adipose Tissue Proteomics.- Quantification of Breast Cancer Protein Biomarkers at Different Expression Levels in Human Tumors.- Quantitative Proteomic Analysis of Mass Limited Tissue Samples for Spatially-resolved Tissue Profiling.- High-throughput Proteomics Analysis of Fresh-frozen Biopsy Tissue Samples using Pressure Cycling Technology Coupled with SWATH Mass Spectrometry.- Straightforward Protocol for Gel-free Proteomic Analysis of Adipose Tissue.- MALDI Imaging Combined to Laser Microdissection-based Microproteomics for Protein Identification. Application to Intratumor Heterogeneity Studies.
| Erscheinungsdatum | 30.09.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1788 |
| Zusatzinfo | 44 Illustrations, color; 6 Illustrations, black and white; XII, 320 p. 50 illus., 44 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | double isotopologue peptide • GeLC-MS • mass limited tissue samples • myofibril-associated proteins • protein quantification • urinary proteomics |
| ISBN-10 | 1-4939-9304-6 / 1493993046 |
| ISBN-13 | 978-1-4939-9304-8 / 9781493993048 |
| Zustand | Neuware |
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