Histone Variants
Humana Press Inc. (Verlag)
978-1-4939-9358-1 (ISBN)
Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.
Methods for Preparing Nucleosomes Containing Histone Variants.- Characterization of Post-Translational Modifications on Histone Variants.- Purification of Histone Variant-Interacting Chaperone Complexes.- Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics.- Histone Native Chromatin Immunprecipitation.- How to Tackle Challenging ChIP-seq, with Long-Range Cross-Linking, using ATRX as an Example.- Time-ChIP : A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance.- MINCE-seq : Mapping In Vivo Nascent Chromatin with EdU and Sequencing.- RChIP-seq : Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes.- Bioinformatics Analysis of Nucleosome and Histone Variant Positioning.- Imaging newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System.- Real Time de novo Deposition of Centromeric Histone-Associated Proteins Usingthe Auxin Inducible Degradation System.- Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin.- CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A.- Studying the Evolution of Histone Variants Using Phylogeny.- Characterization of Post-Meiotic Male Germ Cell Genome Organizational States.- An Animal Model for Genetic Analysis of Multi-Gene Families : Cloning and Transgenesis of Large Tandemly-Repeated Histone Gene Clusters.- Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH).
Erscheinungsdatum | 16.09.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1832 |
Zusatzinfo | 43 Illustrations, color; 19 Illustrations, black and white; XII, 359 p. 62 illus., 43 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Cellular functions • chromatin integrity • Epigenomics • histone chaperones • Nucleosomes |
ISBN-10 | 1-4939-9358-5 / 1493993585 |
ISBN-13 | 978-1-4939-9358-1 / 9781493993581 |
Zustand | Neuware |
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