Schizosaccharomyces pombe

Methods and Protocols

Buch | Softcover

244 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8516-6 (ISBN)

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This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.

Preparation of Solutions and Reagents.- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing.- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe.- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs.- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast.- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast.- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis.- The No-nonsens SDS-PAGE.- Crystallization of Recombinant α-actinin and Related Proteins.- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy.- Antibody Pull-down Experiments in Fission Yeast.- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation.- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo.- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe.- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast.- Random Spore Analysis in Fission Yeast.- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains.- Schizosaccharomyces pombe Biotechnological Applications in Winemaking.- Schizosaccharomyces pombe Isolation Protocol.

Erscheinungsdatum	25.03.2019
Reihe/Serie	Methods in Molecular Biology ; 1721
Zusatzinfo	34 Illustrations, color; 14 Illustrations, black and white; XII, 244 p. 48 illus., 34 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	gel electrophoresis • Immunoprecipitation • microRNA • RNA isolation • snRNA
ISBN-10	1-4939-8516-7 / 1493985167
ISBN-13	978-1-4939-8516-6 / 9781493985166
Zustand	Neuware