Schizosaccharomyces pombe
Humana Press Inc. (Verlag)
978-1-4939-7545-7 (ISBN)
Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.
Preparation of Solutions and Reagents.- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing.- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe.- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs.- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast.- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast.- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis.- The No-nonsens SDS-PAGE.- Crystallization of Recombinant α-actinin and Related Proteins.- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy.- Antibody Pull-down Experiments in Fission Yeast.- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation.- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo.- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe.- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast.- Random Spore Analysis in Fission Yeast.- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains.- Schizosaccharomyces pombe Biotechnological Applications in Winemaking.- Schizosaccharomyces pombe Isolation Protocol.
Erscheinungsdatum | 04.03.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1721 |
Zusatzinfo | 34 Illustrations, color; 14 Illustrations, black and white; XII, 244 p. 48 illus., 34 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Schlagworte | gel electrophoresis • Immunoprecipitation • microRNA • RNA isolation • snRNA |
ISBN-10 | 1-4939-7545-5 / 1493975455 |
ISBN-13 | 978-1-4939-7545-7 / 9781493975457 |
Zustand | Neuware |
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