Rapid Cell Magnetisation Using Cationised Magnetoferritin

Sara Correia Carreira (Autor)

Buch | Softcover

XVII, 166 Seiten

2018 | 1. Softcover reprint of the original 1st ed. 2017
Springer International Publishing (Verlag)
978-3-319-86838-7 (ISBN)

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Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.

Introduction.- Materials and Methods.- Characterising Magnetoferritin and Cationised Magnetoferritin.- Stem Cell Labelling with Cationised Magnetoferritin.- Toxicological Profiling of Cationised Magnetoferritin.- Escherichia coli labelling with Cationised Magnetoferritin.- Overall Conclusions and Future Opportunities.

Erscheinungsdatum	05.03.2022
Reihe/Serie	Springer Theses
Zusatzinfo	XVII, 166 p. 90 illus., 16 illus. in color.
Verlagsort	Cham
Sprache	englisch
Maße	155 x 235 mm
Gewicht	2876 g
Themenwelt	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
Schlagworte	Bacteria Isolation • Bacteria Magnetisation • Magnetic capture of pathogens • Magnetic Cell Separation • Magnetic labelling of biological cells • Magnetic Nanoparticles • Magnetoferritin • Protein modification • Stem Cell Magnetization
ISBN-10	3-319-86838-1 / 3319868381
ISBN-13	978-3-319-86838-7 / 9783319868387
Zustand	Neuware