Rapid Cell Magnetisation Using Cationised Magnetoferritin
Seiten
2017
|
1st ed. 2017
Springer International Publishing (Verlag)
978-3-319-60332-2 (ISBN)
Springer International Publishing (Verlag)
978-3-319-60332-2 (ISBN)
Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
Introduction.- Materials and Methods.- Characterising Magnetoferritin and Cationised Magnetoferritin.- Stem Cell Labelling with Cationised Magnetoferritin.- Toxicological Profiling of Cationised Magnetoferritin.- Escherichia coli labelling with Cationised Magnetoferritin.- Overall Conclusions and Future Opportunities.
| Erscheinungsdatum | 13.07.2017 |
|---|---|
| Reihe/Serie | Springer Theses |
| Zusatzinfo | XVII, 166 p. 90 illus., 16 illus. in color. |
| Verlagsort | Cham |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Gewicht | 444 g |
| Themenwelt | Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik |
| Schlagworte | Bacteria Isolation • Bacteria Magnetisation • Biological and Medical Physics, Biophysics • Biophysics • electricity, electromagnetism & magnetism • Electricity, electromagnetism & magnetism • Genetics (non-medical) • Magnetic capture of pathogens • Magnetic Cell Separation • Magnetic labelling of biological cells • Magnetic Nanoparticles • Magnetism, Magnetic Materials • Magnetoferritin • medical physics • nanochemistry • nanotechnology • Physics • Physics and Astronomy • Protein modification • Stem Cell Magnetization • Stem Cells |
| ISBN-10 | 3-319-60332-9 / 3319603329 |
| ISBN-13 | 978-3-319-60332-2 / 9783319603322 |
| Zustand | Neuware |
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