Membrane Protein Structure and Function Characterization
Humana Press Inc. (Verlag)
978-1-4939-8399-5 (ISBN)
Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2.- Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex.- Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts.- Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits.- Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins.- Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins.- Labeling of Membrane Complexes for Electron Microscopy.- Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor.- Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization.- Secondary Structure Determination by Means of ATR-FTIR Spectroscopy.- Native Mass Spectroscopy forthe Characterization of Structure and Interactions of Membrane Proteins.- Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes.- Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids.- Reconstitution of Membrane Protein in Liposomes.- Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes.- The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein.- Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy.- Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.- Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.- Simulation of Ligand Binding To Membrane Proteins.- Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments.
Erscheinungsdatum | 18.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1635 |
Zusatzinfo | 13 Illustrations, color; 29 Illustrations, black and white; XII, 419 p. 42 illus., 13 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | antibodies • folded protein • functional status • membrane mimicking environments • membrane protein |
ISBN-10 | 1-4939-8399-7 / 1493983997 |
ISBN-13 | 978-1-4939-8399-5 / 9781493983995 |
Zustand | Neuware |
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