Semaphorin Signaling
Humana Press Inc. (Verlag)
978-1-4939-8203-5 (ISBN)
Authoritative and cutting-edge, Semaphorin Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Semaphorins and their Signaling Mechanisms.- Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells.- Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction.- Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression Systems.- Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions.- Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry.- In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin.- Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component.- Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins.- Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins.- Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components.- Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition.- Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse.- An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse.- Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins – A Primary Culture Approach.- Characterizing Semaphorin Signaling Using Isolated Neurons in Culture.- Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues.- Assays to Examine Transmembrane Semaphorin Function In Vitro.- Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth.- Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins.- Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth.- Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones.- Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors.- Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development.- Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling.- Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells.- Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay.- Podocyte Shape Regulation by Semaphorin 3A and MICAL-1.- In Vivo and In Vitro Knockdown Approachesin the Avian Embryo as a Means to Study Semaphorin Signaling.- Assaying the Action of Secreted Semaphorins on Vascular Permeability.- Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis.- Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development.- Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models.- Characterizing Semaphorin Signaling In Vivo Using C. Elegans.
| Erscheinungsdatum | 18.08.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1493 |
| Zusatzinfo | 54 Illustrations, color; 42 Illustrations, black and white; XV, 503 p. 96 illus., 54 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Biochemical • in vitro • in vivo protocols • semaphorins • Tissues |
| ISBN-10 | 1-4939-8203-6 / 1493982036 |
| ISBN-13 | 978-1-4939-8203-5 / 9781493982035 |
| Zustand | Neuware |
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