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Study of Bacteriorhodopsin in a Controlled Lipid Environment - Vivien Yeh

Study of Bacteriorhodopsin in a Controlled Lipid Environment

(Autor)

Buch | Hardcover
139 Seiten
2018 | 1st ed. 2019
Springer Verlag, Singapore
978-981-13-1237-3 (ISBN)
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This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.

Introduction.- Experimental Background.- Effect of Lipid Composition of Nanodisc.- Effect of Size of Nanodisc.- Native Membrane Nanodisc.- Conclusions and Outlook.- References.- Appendix.

Erscheinungsdatum
Reihe/Serie Springer Theses
Zusatzinfo 64 Illustrations, color; 18 Illustrations, black and white; XVIII, 139 p. 82 illus., 64 illus. in color.
Verlagsort Singapore
Sprache englisch
Maße 155 x 235 mm
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Zellbiologie
Naturwissenschaften Chemie Physikalische Chemie
ISBN-10 981-13-1237-0 / 9811312370
ISBN-13 978-981-13-1237-3 / 9789811312373
Zustand Neuware
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