Single-Molecule Studies of Nucleic Acids and Their Proteins

David Bensimon, Vincent Croquette, Jean-François Allemand, Xavier Michalet, Terence Strick (Autoren)

Buch | Hardcover

240 Seiten

2018
Oxford University Press (Verlag)
978-0-19-853092-3 (ISBN)

Artikel merken

This book is an introduction to the novel physical tools that allow single molecule manipulation and visualisation. It describes the use of these tools to understand the elastic properties of single molecules of DNA and RNA and to study their interactions with the many proteins that process them.

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

David Bensimon and Vincent Croquette have been co-leaders of a group at the LPS-ENS that has been studying single DNA molecules and their interactions with proteins since 1991. Jean-François Allemand, Terence Strick and Xavier Michalet have been their students and collaborators ever since. They have jointly developed magnetic traps and have been studying various aspects of single molecule DNA/protein transactions. Since joining the lab of Professor S. Weiss at UCLA , Xavier Michalet has been focusing on the use of fluorescent and spectroscopic methods to study single biomolecules.

1: Introduction to DNA
2: Manipulating DNA
3: Fluorescence Spectroscopy and Microscopy Techniques
4: The Mechanical Properties of Nucleic Acids
5: Structural Transitions in DNA
6: Single Molecule Studies of DNA-associated Motors
7: DNA and RNA Polymerases
8: DNA Helicases
9: Topoisomerases
10: Conclusions

Erscheinungsdatum	10.01.2019
Reihe/Serie	Oxford Graduate Texts
Zusatzinfo	41 grayscale and 45 color line figures; 8 grayscale and 9 color halftone figures
Verlagsort	Oxford
Sprache	englisch
Maße	178 x 249 mm
Gewicht	614 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Chemie ► Organische Chemie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
	Naturwissenschaften ► Physik / Astronomie ► Atom- / Kern- / Molekularphysik
	Naturwissenschaften ► Physik / Astronomie ► Festkörperphysik
ISBN-10	0-19-853092-7 / 0198530927
ISBN-13	978-0-19-853092-3 / 9780198530923
Zustand	Neuware