Polyamines

Methods and Protocols

Rubén Alcázar, Antonio F. Tiburcio (Herausgeber)

Buch | Hardcover

512 Seiten

2017 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-7397-2 (ISBN)

Artikel merken

This volume provides readers with a collection of the latest protocols used by researchers to study polyamines (PA). The chapters in this book cover various topics, such as quantification of different polyamines and conjugates, subcellular localization studies, transport, DNA methylation, ODC regulation, genetic and phenotyping analyses, genome-wide association mapping, polyamine applications and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Polyamines: Methods and Protocols is a useful reference for researchers looking to advance and stimulate their knowledge of polyamines.

Polyamines in the Context of Metabolic Networks.- Molecules for Sensing Polyamines and Transducing their Action in Plants.- Polyamine Metabolism Responses to Biotic and Abiotic Stress.- Thermospermine: An Evolutionary Ancient but Functionally New Compound in Plants.- Determination of Polyamines by Capillary Electrophoresis using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent.- Detection of Thermospermine and Spermine by HPLC in Plants.- Development of Amine-Oxidase Based Biosensors for Spermine and Spermidine Analysis.- Identification of Branched-Chain Polyamines in Hyperthermophiles.- Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry.- Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-Phthalaldehyde.- Determination of Arginine and Ornithine Decarboxylase Activities in Plants.- Determination of S-Adenosylmethionine Decarboxylase Activity in Plants.- Determination of Copper Amine Oxidase Activity in Plant Tissues.- Determination of di-/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay.- Pentamine as a Substrate for Measuring Spermine Oxidase Activity.- Spectrophotometric Quantification of Reactive Oxygen, Nitrogen and Sulfur Species in Plant Samples.- Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay.- Determination of Transglutaminase Activity in Plants.- Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy during Organogenic Nodule Formation in Hop.- Analysis of the Intracellular Localization of Transiently Expressed and Fluorescently Labeled Copper-Containing Amine Oxidases, Diamine Oxidase and N-Methylputrescine Oxidase in Tobacco, using an Agrobacterium infiltration Protocol.- Techniques used for Functional Characterization of Polyamine Transporters.- Quantitative Trait Loci for RootGrowth Response to Cadaverine in Arabidopsis.- Methods Related to Polyamine Control of Cation Transport across Plant Membranes.- Analysis of DNA Methylation Content and Patterns in Plants.- Investigating Ornithine Decarboxylase Post-Transcriptional Regulation via a Pulldown Assay using Biotinylated Transcripts.- Analysis of Cotranslational Polyamine Sensing during Decoding of ODC Antiyme mRNA.- Modulation of Protein Synthesis by Polyamines in Mammalian Cells.- Determination of Post-Translational Modifications by 2D-PAGE: Applications to Polyamines.- Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine Genetics.- Transcriptome Analysis of PA Gain and Loss of Function Mutants.- High-Throughput Phenotyping in Plant Stress Response: Methods and Potential Applications to Polyamine Field.- Abiotic Stress Phenotyping of Polyamine Mutants.- Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes during Pathogenic Interactions.- Real-Time In Vivo Monitoring of Reactive Oxygen Species in Guard Cells.- Genome-Wide Association Mapping Analyses Applied to Polyamines .- Polyamine Metabolism in Climacteric and Non-Climacteric Fruit Ripening.- Application of Polyamines to Maintain Functional Properties in Stored Fruits.- Acrolein: An Effective Biomarker for Tissue Damage Produced from Polyamines.- Polyamines and Cancer.- Potential Applications of Polyamines in Agriculture and Plant Biotechnology.

Erscheinungsdatum	03.11.2017
Reihe/Serie	Methods in Molecular Biology ; 1694
Zusatzinfo	55 Illustrations, color; 39 Illustrations, black and white; XV, 512 p. 94 illus., 55 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	biosynthesis inhibitors • Diamine oxidase • HPLC • Immunocytochemistry • polyamine field
ISBN-10	1-4939-7397-5 / 1493973975
ISBN-13	978-1-4939-7397-2 / 9781493973972
Zustand	Neuware