Detection of Blotted Proteins
Humana Press Inc. (Verlag)
978-1-4939-5595-4 (ISBN)
Western Blotting: Origin and Ascent of the Species.- Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic Evaluation.- Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose.- Detection of Blotted Proteins: Not all Blockers are Created Equal.- Protein Stains to Detect Antigen on Membranes.- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting.- Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation.- Use of Non-Radioactive Detection Method for North- and South-Western Blot.- Immunoblotting using Radiolabeled Reagents for Detection.- Immunoblotting of Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique.- Detection of Protein Carbonyls by Means of Biotin Hydrazide –Streptavidin Affinity Methods.- Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel.- Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays.- Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots.- Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a Standard Proteomics Workflow.- Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and Immunohistochemistry.- Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane.- Sequential use of Immunoblots for Characterization of Autoantibody Specificities.- Nanogold Immunodetection DetectionSystems for the Identification of Autoantigens by Western Blotting.- Application of Intermittent Microwave Irradiation to Western Blot Analysis.- Visualization of Unstained Protein Bands on PVDF.- Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon®-FL Membrane.- Cold Microwave-Enabled Protein Detection and Quantification.- TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics.- Analysis of Electroblotted Proteins by Mass Spectrometry.- On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions.- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore.- Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane.- Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta.- Non-stripping “Rainbow” and Multiple Antigen Detection (MAD) Western Blotting. - Supported Molecular Matrix Electrophoresis.- Parafilm-M®, an Available Cost Effective Alternative for Immuno-blot Pouches.- Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes.- Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva.- A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB.- Other Notable Methods of Membrane Protein Detection: A Brief Review.- Nitrocellulose Membrane: The New Canvas.- Invisible Ink Marking in ECL Membrane Assays.
| Erscheinungsdatum | 11.11.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1314 |
| Zusatzinfo | 48 Illustrations, color; 53 Illustrations, black and white; XIV, 386 p. 101 illus., 48 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | detecting blotted molecular species • detecting the blotted proteins • Protein blotting • Protein transfer • western blotting techniques |
| ISBN-10 | 1-4939-5595-0 / 1493955950 |
| ISBN-13 | 978-1-4939-5595-4 / 9781493955954 |
| Zustand | Neuware |
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