RNA Methodologies
A Laboratory Guide for Isolation and Characterization
Seiten
1993
Academic Press Inc (Verlag)
978-0-12-249700-1 (ISBN)
Academic Press Inc (Verlag)
978-0-12-249700-1 (ISBN)
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A collection of laboratory protocols for the isolation and characterization of mammalian RNA. The book emphasizes how these methodologies can be utilized in the study of transcriptional and post-transciptional regulation of eukaryotic genes.
The isolation of biologically competent and chemically stable RNA is central to molecular biology. As there is currently a need to unify components of RNA characterization, this text presents RNA isolation strategies and a collection of protocols in a coherent start-to-finish format. It is written for anyone capable of conducting basic research, and addresses basic skills involved in nucleic acid research as well as some of the more sophisticated protocols. A collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA, this book focuses on the study of RNA as a parameter of gene expression. "RNA Methodologies: A Laboratory Guide for Isolation and Characterization" emphasizes how these methodologies can be utilized in the study of transcriptional and post-transcriptional regulation of eukaryotic genes. The chapters work together to tell the RNA story, each including clear take-home lessons. The liberal incorporation of flow charts, tables and graphs facilitate learning and assist in the planning phases of a project.
The text studies cellular regulation using RNA as a parameter of gene expression, offers RNA isolation strategies and explains propert handling, storage and manipulation of RNA.
The isolation of biologically competent and chemically stable RNA is central to molecular biology. As there is currently a need to unify components of RNA characterization, this text presents RNA isolation strategies and a collection of protocols in a coherent start-to-finish format. It is written for anyone capable of conducting basic research, and addresses basic skills involved in nucleic acid research as well as some of the more sophisticated protocols. A collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA, this book focuses on the study of RNA as a parameter of gene expression. "RNA Methodologies: A Laboratory Guide for Isolation and Characterization" emphasizes how these methodologies can be utilized in the study of transcriptional and post-transcriptional regulation of eukaryotic genes. The chapters work together to tell the RNA story, each including clear take-home lessons. The liberal incorporation of flow charts, tables and graphs facilitate learning and assist in the planning phases of a project.
The text studies cellular regulation using RNA as a parameter of gene expression, offers RNA isolation strategies and explains propert handling, storage and manipulation of RNA.
RNA and the cellular biochemistry revisited - a rationale; transcription and the organization of eukaryotic gene sequences; messenger RNA; resilient ribonucleases; RNA isolation strategies; isolation of polyadenylated RNA; electrohoresis - principles and parameters; electrophoresis of RNA; the Northern blot; nucleic acid probe technology; practical nucleic acid hybridization; principles of detection; quantititation of specific mRNAs by the S1 nuclease assay; analysis of nuclear RNA; transcription rate assays; extraction of nuclear RNA for steady state analysis; an RNA paradigm.
| Zusatzinfo | appendix, charts, tables, graphs |
|---|---|
| Verlagsort | San Diego |
| Sprache | englisch |
| Maße | 160 x 229 mm |
| Gewicht | 463 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-12-249700-7 / 0122497007 |
| ISBN-13 | 978-0-12-249700-1 / 9780122497001 |
| Zustand | Neuware |
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