Nanoscale Imaging and Characterisation of Amyloid-β (eBook)

Claire Louisa Tinker-Mill (Autor)

eBook Download: PDF

2016 | 1st ed. 2016
XX, 149 Seiten
Springer International Publishing (Verlag)
978-3-319-39534-0 (ISBN)

Infos

This thesis presents a method for reliably and robustly producing samples of amyloid-β (Aβ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide's nanomechanical, elastic, thermal or spectroscopical properties. 

Amyloid-β (Aβ) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of Aβ with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.

Inhalt

Introduction.- Literature Review and Theoretical Concepts.- Experimental Methodology.- Substrate Development of the Imaging of Amyloid Proteins with SPM Methods.- Scanning Probe Microscopy Methods of Imaging Amyloid Peptides During the Aggregation Process.- Spectroscopy and Thermal SPM Methods of Studying Aβ1:42.- The Application of Biophysical Techniques to the Study of the Inhibition of Aggregation of Aβ Using PINPs Liposomes.- Conclusion and Future Perspectives.

Produkt-Details

• Erscheint lt. Verlag: 5.7.2016
• Reihe/Serie: Springer Theses
• Reihe/Serie: Springer Theses
• Zusatzinfo: XX, 149 p. 59 illus., 14 illus. in color.
• Verlagsort: Cham
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Neurologie
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Naturwissenschaften ► Chemie ► Physikalische Chemie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie
• Themenwelt: Technik
• Schlagworte: AFM Imaging of Amyloid-beta • Aggregation of Amyloid-beta • Alzheimer's Disease and AFM • Nanomechanical Characterisation • Ultrasonic Force Microscopy
• ISBN-10: 3-319-39534-3 / 3319395343
• ISBN-13: 978-3-319-39534-0 / 9783319395340