Protein Electrophoresis
Humana Press Inc. (Verlag)
978-1-4939-6241-9 (ISBN)
Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
How It All Began -- A Personal History of Gel-Electrophoresis.- Introduction to Protein Electrophoresis.- Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis.- Protein Solubilization: Attend to the Choice of Lysis Buffer.- Native Polyacrylamide Gels.- Cationic Electrophoresis.- SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins.- Tricine-SDS-PAGE.- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis.- Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins.- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis.- A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples.- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies.- Microchip Electrophoresis, with respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”.- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols.- Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE.- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass.- SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation WesternBlotting.- Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing.- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids.- Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution.- Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE.- One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis.- Two-Dimensional Difference Gel Electrophoresis.- Protein Identification on Archived 2-D Gels.- Diagonal Electrophoresis for the Detection of Protein Disulfides.- Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes.- Immunoelectrophoresis: A Method with Many Faces.- Cell Surface Protein Biotinylation for SDS PAGE Analysis.- A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization.- Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome.- Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test.- Extraction of Proteins from Gels - A Brief Review.- A New Algorithm for the Simulation of SDS 2D-PAGE Datasets.- Isolation of Proteins from Polyacrylamide Gels.- Gel Drying Methods.- Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags.- A Brief Review of Other NotableElectrophoretic Methods.- Protein Stains and Applications.- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels.- Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat.- Silver Staining Techniques of Polyacrylamide Gels.- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis.- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry.- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE.- Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins.- Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping.- Fluorescent Staining of Gels.- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis.- Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain.- Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain.- In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin.- Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black.- Zymographic Assay of Oxidases Using Peroxidase or HeminEntrapped in Polyacrylamide Gels.- Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner.- A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels.- Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein™, ProteoSilver™, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains.- Common Artifacts and Mistakes Made in Electrophoresis.
| Erscheinungsdatum | 30.06.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 869 |
| Zusatzinfo | 31 Illustrations, color; 120 Illustrations, black and white; XIV, 648 p. 151 illus., 31 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Chemie ► Analytische Chemie | |
| Naturwissenschaften ► Chemie ► Physikalische Chemie | |
| ISBN-10 | 1-4939-6241-8 / 1493962418 |
| ISBN-13 | 978-1-4939-6241-9 / 9781493962419 |
| Zustand | Neuware |
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