Plant Cell Biology
A Practical Approach
Seiten
2001
|
2nd Revised edition
Oxford University Press (Verlag)
978-0-19-963865-9 (ISBN)
Oxford University Press (Verlag)
978-0-19-963865-9 (ISBN)
This title covers a range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques.
With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
INTRODUCTION TO OPTICAL MICROSCOPY FOR PLANT CELL BIOLOGY; FLUORESCENT PROBES FOR LIVING PLANT CELLS; FLOW CYTOMETRY; TRANSIENT EXPRESSION, A TOOL TO ADDRESS QUESTIONS IN PLANT CELL BIOLOGY; THE GREEN FLUORESCENT PROTEIN (GFP) AS REPORTER IN PLANT CELLS; MICROINJECTION; MICROMANIPULATION BY LASER MICROBEAM AND OPTICAL TWEEZERS; ELECTROPHYSIOLOGICAL METHODS: MONITORING EXO- AND ENDOCYTOSIS IN REAL TIME; PLANT HISTOLOGY; IMMUNOCYTOCHEMISTRY FOR LIGHT MICROSCOPY; ELECTRON MICROSCOPY; IN SITU HYBRIDIZATION; ORGANELLE ISOLATION
Erscheint lt. Verlag | 31.5.2001 |
---|---|
Reihe/Serie | Practical Approach Series ; 250 |
Zusatzinfo | 4 plates, 18 halftones, 14 line figures |
Verlagsort | Oxford |
Sprache | englisch |
Maße | 189 x 247 mm |
Gewicht | 803 g |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Botanik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 0-19-963865-9 / 0199638659 |
ISBN-13 | 978-0-19-963865-9 / 9780199638659 |
Zustand | Neuware |
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