Laboratory Methods in Enzymology: Protein Part C
Academic Press Inc (Verlag)
978-0-12-420119-4 (ISBN)
TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein–Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates
| Erscheint lt. Verlag | 9.6.2014 |
|---|---|
| Reihe/Serie | Methods in Enzymology |
| Verlagsort | San Diego |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Gewicht | 590 g |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
| Naturwissenschaften ► Biologie ► Biochemie | |
| ISBN-10 | 0-12-420119-9 / 0124201199 |
| ISBN-13 | 978-0-12-420119-4 / 9780124201194 |
| Zustand | Neuware |
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