Proteome Research: Mass Spectrometry

Buch | Softcover

XXI, 274 Seiten

2000 | 1. Softcover reprint of the original 1st ed. 2001
Springer Berlin (Verlag)
978-3-540-67256-2 (ISBN)

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Recent advances in large-scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale idenfification of these proteomes and their modifications. An understanding of these methods is critical for scientists in the "Post-Genome" era.

1 Mass Spectrometry and the Proteome.- 2 Basics of Triple-Stage Quadrupole/Ion-Trap Mass Spectrometry: Precursor, Product and Neutral-Loss Scanning. Electrospray Ionisation and Nanospray Ionisation.- 3 The Basics of Matrix-Assisted Laser Desorption, Ionisation Time-of-Flight Mass Spectrometry and Post-Source Decay Analysis.- 4 Data-Controlled Micro-Scale Liquid Chromatography - Tandem Mass Spectrometry of Peptides and Proteins: Strategies for Improved Sensitivity, Efficiency and Effectiveness.- 5 Solid-Phase Extraction-Capillary Zone Electrophoresis-Mass Spectrometry Analysis of Low-Abundance Proteins.- 6 Protein Identification by Peptide-Mass Fingerprinting.- 7 Protein Identification by SEQUEST.- 8 Interpreting Peptide Tandem Mass-Spectrometry Fragmentation Spectra.- 9 Automated Interpretation of Peptide Tandem Mass Spectra and Homology Searching.- 10 Specific Detection and Analysis of Phosphorylated Peptides by Mass Spectrometry.- 11 Glycoproteomics: High-Throughput Sequencing of Oligosaccharide Modifications to Proteins.- 12 Proteomics Databases.- 13 Quo Vadis.

Erscheint lt. Verlag	26.10.2000
Reihe/Serie	Principles and Practice
Zusatzinfo	XXI, 274 p.
Verlagsort	Berlin
Sprache	englisch
Maße	155 x 235 mm
Gewicht	446 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	2D Gel Analysis • Cell • Chromatography • Database Analysis • DNA • DNA sequencing • Genome • Laser • Mass Spectrometry • Oligosaccharid • Oligosaccharide • Protein • Protein Identification • proteins • Proteom • Proteomics • sequencing • Spektrometrie
ISBN-10	3-540-67256-7 / 3540672567
ISBN-13	978-3-540-67256-2 / 9783540672562
Zustand	Neuware