Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

T. Rabilloud (Herausgeber)

Buch | Softcover

XVI, 248 Seiten

1999 | 2000
Springer Berlin (Verlag)
978-3-540-65792-7 (ISBN)

Infos

Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.

Inhalt

Solubilization of proteins in 2D electrophoresis.- 2D electrophoresis with carrier ampholytes.- 2D electrophoresis with immobilized pH gradients.- Detection of proteins on 2D electrophoresis gels.- Blotting and immunoaffinity identification of 2-DE separated proteins.- Identification of proteins by amino acid composition after acid hydrolysis.- Identification by amino acid composition obtained from labeling.- Identification of proteins by amino acid sequencing.- Identification of proteins by mass spectrometry.- Mass spectrometry of intact proteins from two-dimensional PAGE.

Produkt-Details

• Erscheint lt. Verlag: 17.9.1999
• Reihe/Serie: Principles and Practice
• Zusatzinfo: XVI, 248 p.
• Verlagsort: Berlin
• Sprache: englisch
• Maße: 155 x 235 mm
• Gewicht: 410 g
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Schlagworte: 2D electrophoresis • 2D Elektrophorese • Amino acid • amino acid sequencing • Aminosäure-Sequenzierung • Chemistry • Elektrophorese • genes • Genome • Membrane • Polyacrylamid-Gelelektrophorese • Protein • protein dectection • Protein-Massenspektrometrie • protein mass spectrometry • Protein-Nachweis • proteins • Protein-Sequenzierung • Proteom • Proteome • Proteomics • Säure • sequencing • Sequenzierung • termination
• ISBN-10: 3-540-65792-4 / 3540657924
• ISBN-13: 978-3-540-65792-7 / 9783540657927
• Zustand: Neuware