A Guide to Methods in the Biomedical Sciences (eBook)

Ronald B. Corley (Autor)

eBook Download: PDF

2006 | 2005
XVI, 126 Seiten
Springer US (Verlag)
978-0-387-22845-7 (ISBN)

Infos

A Guide to Methods in the Biomedical Sciences gives a basic description of common methods used in research. This is not intended to be a methods book. Rather, it is intended to be a book that outlines the purpose of the methods described, their limitations and provide alternative approaches as appropriate. Thousands of methods have been developed in the various biomedical disciplines and those covered in this book represent the basic, essential and most widely used methods in several different disciplines. The historical background (including some interesting anecdotes) leading to the development of ground-breaking techniques is described, especially of those that significantly advanced the field of biomedical research. Advances that earned their inventors prestigious Nobel Prizes are emphasized.The book is divided into six sections, highlighting selected methods in protein chemistry, nucleic acids, recombinant DNA technology (including forensic-based methods), antibody-based techniques, microscopy and imaging, and the use of animals in biomedical sciences.

Inhalt

1: Detection And Analysis Of Proteins
A. Introduction
B. Basic methods for protein analysis
C. Characterization of primary, secondary, tertiary
and quaternary structures of proteins

2: Detection And Analysis Of Nucleic Acids
A. Introduction
B. Basic methods for nucleic acid analysis
C. DNA sequencing
D. Detection and quantitation of RNA

3: Recombinant DNA Techniques: Cloning And Manipulation Of DNA
A. Introduction
B. Plasmid and viral vectors
C. Libraries
D. Site Directed Mutagenesis
E. Polymerase chain reaction (PCR)
F. Screening for differentially expressed genes (DEGs)
G. Promoter-protein interactions
H. Silencing gene expression
I. Forensics and DNA technology

4: Antibody-Based Techniques
A. Introduction
B. Monoclonal antibodies
C. Purification and use of antibodies
D. Flow cytometry and fluorescence activated cell
sorting (FACS)
E. Other assays using antibodies

5: Microscopy: Imaging Of Biological Specimens
A. Introduction
B. Light microscopy
C. Fluorescence and laser confocal microarray microscopy
D. Electron Microscopy (EM)
E. Magnetic resonance imaging (MRI)

6: The Derivation And Manipulation Of Experimental
Animals In Biomedical Sciences
A. Introduction
B. Inbred strains of mice
C. Congenic strains of mice
D. Transgenic mice
E. Targeted transgenes: 'knockout' and 'knockin' mice
F. Other uses of mice in biomedical research

References
Some excellent methodology manuals
Index

Produkt-Details

• Erscheint lt. Verlag: 16.1.2006
• Zusatzinfo: XVI, 126 p.
• Verlagsort: New York
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Allgemeines / Lexika
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Biomedizin
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Schlagworte: DNA • DNA sequencing • electron microscopy • gene expression • genes • Methodology • microarray • Microscopy • PCR • Promoter
• ISBN-10: 0-387-22845-4 / 0387228454
• ISBN-13: 978-0-387-22845-7 / 9780387228457