Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use (eBook)

Volume 1

Elizabeth H. Harris (Autor)

Elizabeth H Harrisduke (Herausgeber)

eBook Download: EPUB

2009 | 2. Auflage
480 Seiten
Elsevier Science (Verlag)
978-0-08-091955-3 (ISBN)

Infos

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. - Expanded revision of gold standard reference - Includes latest advances in research, including completion of the genome - Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields - Available as part of a 3-Volume Set or sold individually

Inhalt

Front Cover 1
The Chlamydomonas Sourcebook 4
Copyright Page 5
Contents of Volume 1 6
Preface 10
Acknowledgments 12
Conventions Used 14
Abbreviations 16
List of Tables 18
List of Figures 20
Contents of Volume 2 24
Contents of Volume 3 34
CHAPTER 1 The Genus Chlamydomonas 40
I. Introduction 40
II. Description of the genus 40
III. Chlamydomonas genetics: 1830–1960 48
IV. Origins of the major laboratory strains of C. reinhardtii 50
V. Other isolates of C. reinhardtii 55
VI. Other Chlamydomonas species used experimentally 57
CHAPTER 2 Cell Architecture 64
I. Introduction 64
II. Early ultrastructural studies 66
III. The cell wall 66
IV. Basal bodies, flagellar roots, and cellular filaments 83
V. The nucleus 88
VI. Golgi and endoplasmic reticulum 89
VII. Contractile vacuoles 90
VIII. Microbodies and other membrane-bound structures 92
IX. Mitochondria 93
X. The chloroplast 96
XI. The eyespot 99
CHAPTER 3 Cell Division 104
I. Introduction 104
II. Mitosis 104
III. Meiosis 113
IV. Chromosome cytology 115
V. The cell cycle 117
CHAPTER 4 Motility and Behavior 128
I. Introduction 128
II. Flagellar structure and organization 129
III. Assembly and maintenance of the flagella 138
IV. Motility 144
V. Cell behavior 147
CHAPTER 5 The Sexual Cycle 158
I. Introduction 158
II. Types of sexual reproduction within the genus Chlamydomonas 159
III. Stages in the reproductive process 169
IV. Vegetative diploids and cytoduction 191
V. Genetic control of sexuality 192
VI. Perturbations of the mating process 194
CHAPTER 6 The Life of an Acetate Flagellate 198
I. Introduction 199
II. Nucleic acids and protein synthesis 199
III. Metabolic processes 215
IV. Photosynthesis 236
V. Respiration 245
VI. Hydrogen production 247
CHAPTER 7 Organelle Heredity 250
I. Introduction 250
II. Uniparental inheritance 251
III. Organelle DNA 267
IV. RNA and protein synthesis in the chloroplast 274
V. Coordination of nuclear and organelle genomes in organelle biogenesis 277
CHAPTER 8 Chlamydomonas in the Laboratory 280
I. Introduction 281
II. Culture media 281
III. Culture conditions 287
IV. Harvesting and fractionating cultures 299
V. Histological techniques 299
VI. Isolation of Chlamydomonas species from nature 300
VII. Storage of Chlamydomonas strains 301
VIII. Genetic analysis 306
IX. The molecular toolkit 331
APPENDIX: Resources for the Investigator 342
References 348
Index 474
A 474
B 474
C 474
D 476
E 476
F 476
G 476
H 476
I 477
K 477
L 477
M 477
N 478
O 478
P 478
Q 479
R 479
S 479
T 479
U 480
V 480
W 480
X 480
Y 480
Z 480
Index to Chlamydomonas species other than C. reinhardtii 482
A 482
B 482
C 482
D 482
E 482
F 482
G 482
H 482
I 482
K 482
L 482
M 482
N 483
O 483
P 483
R 483
S 483
T 483
U 483
V 483
Y 483
Z 483

Produkt-Details

• Erscheint lt. Verlag: 7.3.2009
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Botanik
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Technik
• ISBN-10: 0-08-091955-3 / 0080919553
• ISBN-13: 978-0-08-091955-3 / 9780080919553