Regulation of Gene Expression (eBook)

Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters (Autoren)

eBook Download: PDF

2008 | 2007
IX, 333 Seiten
Humana Press (Verlag)
978-1-59745-228-1 (ISBN)

Infos

The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select 'Courses. ' The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

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The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select "e;Courses. "e; The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Inhalt

Preface 5
Contents 7
COMPANION CD 9
Part I GENE EXPRESSION CONTROL AT THE MRNA LEVEL 10
Messenger RNA Accumulation 20
1. Concepts 20
2. Basic Methods and Approaches 23
3. Summary 38
Transcript Profiling 40
1. Concepts 40
2. Methods and Approaches 42
3. Summary 57
Transcriptional Regulation of Gene Expression 58
1. Concepts 58
2. Methods and Approaches 69
3. Summary 107
Posttranscriptional Processing of Messenger RNA 114
1. Concepts 114
2. Methods and Approaches 125
3. Summary 145
Study Questions 150
References 152
Part II REGULATION OF PROTEIN LEVELS AND TRANSCRIPTION FACTOR FUNCTION 156
Overview 158
Protein Synthesis and Turnover 164
1. Concepts 164
2. Methods and Approaches 166
3. Summary 168
Proteomics 170
1. Concepts 170
2. Methods and Approaches 171
3. Summary 181
Determination of Protein–Protein Interactions and the Motifs That Mediate Them 182
1. Concepts 182
2. Methods and Approaches 182
3. Summary 223
Posttranslational Modifications 224
1. Concepts 224
2. Methods and Approaches 229
3. Summary 246
Dissection of Signaling Pathways 248
1. Concepts 248
2. Methods and Approaches 249
3. Summary 266
Study Questions 268
References 270
Part III USE OF TRANSGENIC AND KNOCKOUT MICE TO STUDY GENE REGULATION 274
OVERVIEW 276
Production of Transgenic Animals 280
1. Standard Production 280
2. Applications of Transgenic Models 287
KNOCKOUT ANIMALS 304
1. Standard Production 304
2. Applications of Null Mouse Models 309
Isolation of Cells and Cell Lines From Transgenic and Knockout Animals 328
1. Standard Production 328
Study Questions 330
References 332
Index 338

Produkt-Details

• Erscheint lt. Verlag: 17.8.2008
• Zusatzinfo: IX, 333 p.
• Verlagsort: Totowa
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Technik
• Schlagworte: cell lines • gene expression • genes • Molecular mechanisms • proteins • Proteomics • RNA • termination • transcription • Transgenic animals • Translation
• ISBN-10: 1-59745-228-9 / 1597452289
• ISBN-13: 978-1-59745-228-1 / 9781597452281