Proteomics of Human Body Fluids (eBook)

Principles, Methods, and Applications

Visith Thongboonkerd (Herausgeber)

eBook Download: PDF

2008 | 2007
XIII, 533 Seiten
Humana Press (Verlag)
978-1-59745-432-2 (ISBN)

Infos

This volume is the first collection of applications of proteomics to analyze various human body fluids. Proteomics of Human Bodyfluids consists of two parts. The first provides basic principles and strategies for proteomic analysis of human body fluids. The second offers more details regarding methodologies and recent findings and clinical applications of each specific type of human body fluids.

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Rapid growth of the "e;proteomics"e; field during the past twelve years has c- tributed to significant advances in science. To date, proteomic technologies have been widely applied to examining various kinds of biological materials. Clinical proteomics is the concept of using proteomic techniques to evaluate the proteomes in clinical samples for biomarker discovery and for better understa- ing of normal physiology and pathogenic mechanisms of human diseases. Tra- lating the proteomic information to clinical practice may lead to the ultimate goals of earlier diagnosis, improved therapeutic outcome and successful prev- tion of diseases. Human body fluids are produced, secreted, and/or excreted from various tissues or organs. Major compositions of body fluids are water, organic s- stances and inorganic compounds. These compositions vary in each body fluid, making one's function different from the others. Analyses of protein com- nents in individual fluids would increase current knowledge on the biology and physiology of various organ systems, and on the pathophysiology of diseases, which cause alterations in protein production, secretion, and/or excretion from the affected tissues or organs into the body fluids. Additionally, human body fluids are the main targets and valuable sources for biomarker discovery. As the high-throughput capability and applicability of proteomics for analyzing proteins in the body fluids have been already proven, these desires are most likely achievable by using proteomic technologies.

Inhalt

Preface 5
Contents 7
Contributors 9
I PRINCIPLES OF PROTEOMICS APPLIED TO HUMAN BODY FLUIDS 14
Proteomic Strategies for Analyzing Body Fluids 15
Sample Preparation of Body Fluids for Proteomics Analysis 43
Multiplexed Immunoassays for Protein Profiling in Human Body Fluids 82
Deciphering the Hieroglyphics of Functional Proteomics Using Small Molecule Probes 94
Modification-Specific Proteomic Analysis of Glycoproteins in Human Body Fluids by Mass Spectrometry 117
Plasma Proteome Database 139
2D PAGE Databases for Proteins in Human Body Fluids 147
Bioinformatics and Experimental Design for Biomarker Discovery 157
Integrative Omics, Pharmacoproteomics, and Human Body Fluids 185
II PROTEOMIC ANALYSIS OF SPECIFIC TYPES OF HUMAN BODY FLUIDS 203
The Human Plasma and Serum Proteome 204
Proteomics of Human Urine 234
Proteomics of Human Cerebrospinal Fluid 278
Proteomics of Pleural Effusion 294
Proteomics of Bronchoalveolar Lavage Fluid and Sputum 317
Proteomics of Sinusitis Nasal Lavage Fluid 334
Proteomics of Human Saliva 354
Proteomics of Human Pancreatic Juice 384
Proteomics of Human Bile 405
Proteomics of Amniotic Fluid 421
Proteomics of Human Milk 443
Proteomics of Nipple Aspirate Fluid in Nonlactating Women 458
Proteomics of Seminal Fluid 472
Proteomics of Vitreous Fluid 499
Proteomics of Human Dialysate and Ultrafiltrate Fluids Yielded by Renal Replacement Therapy 512
Index 524

Produkt-Details

• Erscheint lt. Verlag: 23.1.2008
• Zusatzinfo: XIII, 533 p. 103 illus., 7 illus. in color.
• Verlagsort: Totowa
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Anamnese / Körperliche Untersuchung
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Technik
• Schlagworte: Bioinformatics • Endoplasmatisches Reticulum • glycoprotein • Polyacrylamid-Gelelektrophorese • proteins • Proteomics
• ISBN-10: 1-59745-432-X / 159745432X
• ISBN-13: 978-1-59745-432-2 / 9781597454322