Forensic DNA Analysis
Springer-Verlag New York Inc.
978-1-0716-3297-0 (ISBN)
This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Forensic DNA Analysis: An Overview of the Laboratory Process.- Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods.- Manual Silica-Based DNA Extractions.- Applied Biosystems(TM) PrepFiler(TM) Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express(TM)).- Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation.- DNA Extraction of Bone through Demineralization.- Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ(TM) Methods.- DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards.- Yield Gel via Quantitative Gel Electrophoresis.- Quantitative PCR of Alu Repeats using PowerUp(TM) SYBR® Green Master Mix.- Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit.- QIAGEN's Investigator® Quantiplex Pro Kit.- DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C).- Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System.- Applied Biosystems' GlobalFiler(TM) PCR Amplification Kit.- QIAGEN's Investigator® 24plex QS and GO! PCR Amplification.- Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis.- Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer.- Likelihood Ratio Calculation using LRmix Studio.- Mitochondrial DNA Analysis.- An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints.- Detection of Latent DNA using a DNA Binding Dye.- Rapid DNA Profile Development with Applied Biosystems RapidHIT(TM) ID System.- Next-Generation Sequencing: ForenSeq(TM) DNA Signature Prep Kit with the Illumina MiSeq FGx.
| Erscheinungsdatum | 16.07.2024 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 92 Illustrations, color; 3 Illustrations, black and white; IX, 431 p. 95 illus., 92 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Rechtsmedizin |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Schlagworte | Bioinformatics • DNA amplification • DNA analysis • DNA Extraction • DNA profiling |
| ISBN-10 | 1-0716-3297-3 / 1071632973 |
| ISBN-13 | 978-1-0716-3297-0 / 9781071632970 |
| Zustand | Neuware |
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