Forensic DNA Analysis

Methods and Protocols

Buch | Hardcover

431 Seiten

2023 | 2023 ed.
Springer-Verlag New York Inc.
978-1-0716-3294-9 (ISBN)

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This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.

Forensic DNA Analysis: An Overview of the Laboratory Process.- Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods.- Manual Silica-Based DNA Extractions.- Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™).- Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation.- DNA Extraction of Bone through Demineralization.- Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods.- DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards.- Yield Gel via Quantitative Gel Electrophoresis.- Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix.- Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit.- QIAGEN's Investigator® Quantiplex Pro Kit.- DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C).- Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System.- Applied Biosystems' GlobalFiler™ PCR Amplification Kit.- QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification.- Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis.- Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer.- Likelihood Ratio Calculation using LRmix Studio.- Mitochondrial DNA Analysis.- An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints.- Detection of Latent DNA using a DNA Binding Dye.- Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System.- Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.

Erscheinungsdatum	18.07.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	92 Illustrations, color; 3 Illustrations, black and white; IX, 431 p. 95 illus., 92 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Rechtsmedizin
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	Bioinformatics • DNA amplification • DNA analysis • DNA Extraction • DNA profiling
ISBN-10	1-0716-3294-9 / 1071632949
ISBN-13	978-1-0716-3294-9 / 9781071632949
Zustand	Neuware