Targeted Protein Degradation
Springer-Verlag New York Inc.
978-1-0716-1664-2 (ISBN)
Authoritative and cutting-edge, Targeted Protein Degradation: Methods and Protocols aims to ensure successful results in this emerging field of drug discovery.
Protein Ligand Interactions Using Surface Plasmon Resonance.- High-Throughput Detection of Ligand-Protein Binding using a SplitLuc Cellular Thermal Shift Assay.- Proteins and their Interacting Partners: An Introduction to Protein-Ligand Binding Site Prediction Methods with a Focus on FunFOLD3.- Evaluating ligands for ubiquitin ligases using affinity beads.- Mechanistic and Structural Features of PROTAC Ternary Complexes.- MST and TRIC Technology to Reliably Study PROTAC Binary and Ternary Binding In Drug Development.- An in vitro Pull-down Assay of the E3 ligase:PROTAC:Substrate Ternary Complex to Identify Effective PROTACs.- Kinetic detection of E3: PROTAC: Target Ternary Complexes using NanoBRET technology in Live Cells.- Inducing Ubiquitylation and Protein Degradation as a Drug Development Strategy.- Methodological Versatility of Tandem Ubiquitin Binding Entities (TUBEs) for Understanding Poly-ubiquitination and its Various Chains.- Global Mass Spectrometry-Based Analysis of Protein Ubiquitination Using K-e-GG Remnant Antibody Enrichment.- Determination of Proteasomal Unfolding Ability.- Methods for Quantitative Assessment of Protein Degradation.- A High Throughput method to prioritize PROTAC intracellular target engagement and cell permeability using NanoBRET.- Profiling CELMoD Mediated Degradation of Cereblon Neosubstrates.- Global Proteome Profiling to Assess Changes in Protein Abundance using Isobaric Labeling and Liquid Chromatography Tandem Mass Spectrometry.- PHOTACs Enable Optical Control of Protein Degradation.- Protocols for synthesis of SNIPERs and the methods to evaluate the anti-cancer affects.
Erscheinungsdatum | 03.09.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2365 |
Zusatzinfo | 50 Illustrations, color; 17 Illustrations, black and white; XI, 351 p. 67 illus., 50 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Pharmazie |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Chemie ► Technische Chemie | |
Technik | |
ISBN-10 | 1-0716-1664-1 / 1071616641 |
ISBN-13 | 978-1-0716-1664-2 / 9781071616642 |
Zustand | Neuware |
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