Human Gametes and Preimplantation Embryos (eBook)

Assessment and Diagnosis

David K. Gardner, Denny Sakkas, Emre Seli, Dagan Wells (Herausgeber)

eBook Download: PDF

2013 | 2013
XV, 306 Seiten
Springer New York (Verlag)
978-1-4614-6651-2 (ISBN)

Infos

In recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates.

Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of 'omics' approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine.

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In recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates. Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of "e;omics"e; approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine.

Inhalt

1. Current Practices and Regulations for Embryo Transfer Worldwide: Implications on IVF Outcome Baris Ata and Emre Seli2. Analysis of the Zona Pellucida as an Indicator of Oocyte Developmental PotentialMarkus Montag, Thomas Schimming, and Maria Köster3. Morphological Assessment of Oocytes, Pronuclear and Cleavage Stage Embryos Laura Rienzi, Benedetta Iussig, and Filippo Ubaldi4. Morphological Assessment of Blastocyst Stage Embryos: Types of Grading Systems and Their Reported OutcomesBasak Balaban and David K. Gardner5. Real Time Imaging Strategies to Improve Morphological AssessmentMaría Cruz, Manuel Muñoz, and Marcos Meseguer6. Limitations and Benefits of Morphologic Embryo Assessment Strategies: How Far Can Morphological Assessment Go in the Identification of Viable Embryos?Denny Sakkas and David K. Gardner7. Sperm Morphologic Characteristics and Their Impact on Embryo Quality and Pregnancy OutcomeBhushan K. Gangrade and Ashok Agarwal8. Objective Biomarkers of Sperm Development and Fertility: Assessment of Sperm-Zona Pellucida Binding Ability and Hyaluronic Acid Mediated Selection of Sperm for ICSI Fertilization Gabor Huszar 9. Selecting the Best Sperm and Its Implications in Clinical PracticeJuan G. Alvarez10. The Origins of Aneuploidy in Human EmbryosElpida Fragouli and Joy Delhanty11. Preimplantation Genetic Screening and Diagnosis Using Fluorescent In Situ Hybridization (FISH)Tsilya Gerasimova and Maria D. Lalioti 12. Single Nucleotide Polymorphisms and Next Generation SequencingAlan H. Handyside and Dagan Wells13. Use of Comparative Genomic Hybridization (CGH) and Microarray-CGH for Preimplantation Genetic ScreeningLeeanda Wilton and Dagan Wells 14. Quantitative SNP Array and Real-Time PCR Based Human Preimplantation Embryo Aneuploidy Screening Nathan R. Treff15. Regulation of Gene Expression in the Oocyte and Early Embryo: Implications for Transcriptomic and Proteomic Embryo AssessmentHelena Kristiansson and Emre Seli16. Transcriptomics Technology: Promise and Potential Pitfalls Can Bruce and Asli Uyar17. Transcriptomic Analysis of Cumulus and Granulosa Cells as a Marker of Embryo ViabilityElpida Fragouli  and Dagan Wells18. Mass Spectrometry, Proteomics and the Study of Sperm Cell BiologyMark A. Baker and R. John Aitken19. Proteomic Analysis of Embryo Viability Mandy G Katz-Jaffe and Susanna McReynolds20. Metabolism of the Viable Human EmbryoDavid K. Gardner21. Analysis of Respiration as an Indicator of Oocyte and Embryo Developmental Potential David L. Keefe22. Mitochondrial Activity as a Biomarker of Gamete and Embryo Health Jonathan Van Blerkom23. Carbohydrate Analysis and Embryo ViabilityMichelle Lane and David K. Gardner24. Identification of Viable Embryos by Non-invasive Measurement of Amino Acids in Culture MediaFranchesca D. Houghton25. Metabolomic Profiling of Embryos Using SpectroscopyDenny Sakkas and Emre Seli26. Microfluidic Devices for the Analysis of Gamete and Embryo Physiology George A. Thouas, David L. Potter, and David K. Gardner

Produkt-Details

• Erscheint lt. Verlag: 27.5.2013
• Zusatzinfo: XV, 306 p. 64 illus., 48 illus. in color.
• Verlagsort: New York
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Gynäkologie / Geburtshilfe
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Histologie / Embryologie
• Schlagworte: ART • Artificial Insemination • assisted reproduction • genomics • Infertility • In vitro fertilization • IVF • Metabolomics • Proteomics
• ISBN-10: 1-4614-6651-2 / 1461466512
• ISBN-13: 978-1-4614-6651-2 / 9781461466512