Molecular Aspects of Hematologic Malignancies (eBook)

Diagnostic Tools and Clinical Applications

Michal Witt, Malgorzata Dawidowska, Tomasz Szczepanski (Herausgeber)

eBook Download: PDF

2012 | 2012
X, 476 Seiten
Springer Berlin (Verlag)
978-3-642-29467-9 (ISBN)

Infos

This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors' own laboratories.

Inhalt

Part I  MOLECULAR BIOLOGY OF SELECTED HEMATO-ONCOLOGIC DISEASES 1.        Molecular biology of acute lymphoblastic leukemia Małgorzata Dawidowska, Monika  D. Kraszewska, Katarzyna Derwich, and Tomasz Szczepański 2.        Genetic mechanisms and molecular markers of neoplastic transformation in acute myeloid leukemiaAgata A. Filip, Marta Libura, Sebastian Giebel, and Olga Haus3.        Molecular pathogenesis of aggressive B-cell lymphomasPrzemysław Juszczyński and Krzysztof Warzocha4.        Molecular biology and classification of multiple myelomaAnna Dmoszyńska and Norbert Grząśko5.        Chronic lymphocytic leukemiaAnton W. Langerak and Yorick Sandberg 6.        The molecular biology of chronic myeloid leukemia Tomasz Sacha, Kajetana Foryciarz, and Aleksander B. Skotnicki 7.        Molecular biology of myelodysplastic syndromesFrancesc Solé8.        Myeloproliferative neoplasmsAndrzej Hellmann, Maria Bieniaszewska, Witold Prejzner, and Aleksandra Leszczyńska  Part II  DIAGNOSTICS AND MONITORING OF THERAPY OF HEMATO-ONCOLOGIC DISEASES 9.        Flow cytometric immunophenotyping as diagnostic tool of hematopoietic malignanciesŁukasz Sędek, Juan Flores-Montero, Joanna Bulsa, Susana Barrena, Julia Almeida, Alberto Orfao, and Tomasz Szczepański 10.     Cytogenetics in hematologyOlga Haus, Anna Poluha, and Katarzyna Skonieczka11.     Monitoring of minimal residual disease in acute lymphoblastic leukemiaTomasz Szczepański, Małgorzata Dawidowska, and Katarzyna Derwich12.     Gene expression profiling in hematologic malignanciesPrzemysław Juszczyński, Bjoern Chapuy, Małgorzata Szczepaniak, and Krzysztof Warzocha Part III  MOLECULAR PROCEDURES INVOLVED IN HEMATOPOIETIC STEM CELL TRANSPLANTATION 13.     Allogeneic transplantation of hematopoietic stem cellsJacek Wachowiak14.     Genetic basis of donor-recipient matching in allogeneic transplantation of hematopoietic stem cells Jacek Nowak and Jacek Wachowiak15.     Chimerism following allogeneic transplantation of hematopoietic stem cellsMałgorzata Dawidowska, Katarzyna Guz, Ewa Brojer, Jacek Wachowiak, andMichał Witt16.     Peritransplantation monitoring of minimal residual disease in acute lymphoblastic leukemiaMałgorzata Dawidowska, Katarzyna Derwich, and Tomasz Szczepański17.     Biobanks of cellular materialWiesław Wiktor-Jedrzejczak and Elzbieta Urbanowska Part IV  MOLECULAR DIAGNOSTIC PROTOCOLS 18.     Biobanking of cellular materialAnna Poluha and Elżbieta Urbanowska19.     Isolation of mononuclear cells from human blood and bone marrow by density gradient centrifugationMałgorzata Dawidowska20.     Molecular techniques commonly used in hematooncolologyMonika D. Kraszewska and Ewa Ziętkiewicz21.     Genetic methods of HLA typingJacek Nowak, Renata Mika-Witkowska, and Elżbieta Graczyk-Pol22.     Post-transplant chimerism analysis through STR-PCR and RQ-PCRMałgorzata Dawidowska, Katarzyna Guz,  Agnieszka Orzińska, Justyna Smolarczyk-Wodzyńska, Monika D. Kraszewska, and Michał Witt23.     Analysis of minimal residual disease with the use of rearrangements of Ig/TCR genesthrough RQ-PCRM. Dawidowska, Vincent H. J. van der Velden, Michał Witt, and Tomasz Szczepański24.     Molecular diagnostics of acute myeloblastic leukemiaMarta Libura, Agata A. Filip, and  Olga Haus25.     Assessment of the presence and the level of BCR-ABL fusion gene expression and mutational status in ABL kinase domain.Sylwia Czekalska, Magdalena Zawada, and Izabela Florek 26.     Studies of rearrangements and somatic hypermutation of IGHV genes in chronic lymphocytic leukemiaAnton W. Langerak, Richard Rosenquist, Paolo Ghia, Chrysoula Belessi, Kostas Stamatopoulos, and Frederic Davi  for the ERIC-IgCLL group27.     Molecular biology methods in the diagnosis of multiple myelomaAnna Dmoszyńska and Sylwia Chocholska28.     Molecular methods in myeloproliferative neoplasmsAleksandra Leszczyńska, Witold Prejzner, Maria Bieniaszewska, and Andrzej Hellmann29.     Informed consent for participation in research project Małgorzata Dawidowska and Michał Witt 

Produkt-Details

• Erscheint lt. Verlag: 30.6.2012
• Reihe/Serie: Principles and Practice
• Reihe/Serie: Principles and Practice
• Zusatzinfo: X, 476 p.
• Verlagsort: Berlin
• Sprache: englisch
• Original-Titel: Hematologia Molekularna: patogeneza, patomechanizmy i metody badawcze
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Innere Medizin
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
• Schlagworte: Hematology • leukemia • minimal residual disease • Molecular Biology • Oncology • stem cell transplantation
• ISBN-10: 3-642-29467-7 / 3642294677
• ISBN-13: 978-3-642-29467-9 / 9783642294679