Protein Sensors and Reactive Oxygen Species, Part B: Thiol Enzymes and Proteins (eBook)

Lester Packer, Helmut Sies (Herausgeber)

eBook Download: PDF

2002 | 1. Auflage
404 Seiten
Elsevier Science (Verlag)
978-0-08-049696-2 (ISBN)

Infos

This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.

Inhalt

Cover 1
Table of Contents 6
Contributors to Volume 348 10
Preface 14
Volume in Series 16
Chapter 1. Thiols in Redox Mechanism of Ribonucleotide Reductase 36
Chapter 2. Tyrosyl Radicals and Ribonucleotide Reductase 56
Chapter 3. Flavin-Dependent Sulfhydryl Oxidases in Protein Disulfide Bond Formation 65
Chapter 4. Analyzing Cotranslational Protein Folding and Disulfide Formation by Diagonal Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis 70
Chapter 5. Disulfide Reduction in Major Histocompatibility Complex Class II-Restricted Antigen Processing by Interferon-.-Inducible Lysosomal Thiol Reductase 78
Chapter 6. Thiol Oxidation and Reduction in Major Histocompatibility Complex Class I-Restricted Antigen Processing and Presentation 84
Chapter 7. Disulfide Bond Formation in Periplasm Escherichia coli 89
Chapter 8. Protein Disulfide Isomerase as an Enzyme and a Chaperone in Protein Folding 101
Chapter 9. Characterization of Redox-Active Proteins on Cell Surface 111
Chapter 10. Measurement of Reduction of Disulfide Bonds in Plasmin by Phosphoglycerate Kinase 122
Chapter 11. Redox Potential of GSH/GSSG Couple: Assay and Biological Significance 128
Chapter 12. Role of Yeast Flavin-Containing Monooxygenase in Maintenance of Thiol–Disulfide Redox Potential 148
Chapter 13. Identification of Cysteine Sulfenic Acid in AhpC of Alkyl Hydroperoxide Reductase 157
Chapter 14. Glutaredoxins and Oxidative Stress Defense in Yeast 171
Chapter 15. Quantitation of Protein Sulfinic and Sulfonic Acid, Irreversibly Oxidized Protein Cysteine Sites in Cellular Proteins 181
Chapter 16. c-Jun Regulation by S-Glutathionylation 192
Chapter 17. S-Glutathionylation of Glyceraldehyde-3-phosphate Dehydrogenase: Role of Thiol Oxidation and Catalysis by Glutaredoxin 210
Chapter 18. Roles of Nrf2 in Activation of Antioxidant Enzyme Genes via Antioxidant Responsive Elements 217
Chapter 19. Enzymatic Pathways of ß Elimination of Chemopreventive Selenocysteine Se Conjugates 226
Chapter 20. Gene Expression and Thiol Redox State 235
Chapter 21. Redox Flow as an Instrument of Gene Regulation 251
Chapter 22. Optical Methods for Measuring Zinc Binding and Release, Zinc Coordination Environments in Zinc Finger Proteins, and Redox Sensitivity and Activity of Zinc-Bound Thiols 265
Chapter 23. Metallothionein Expression and Oxidative Stress in the Brain 273
Chapter 24. Reversible Oxidation of HIV-2 Protease 284
Chapter 25. Thiol Enzymes Protecting Mitochondria against Oxidative Damage 295
Chapter 26. Phenylarsine Oxide Affinity Chromatography to Identify Proteins Involved in Redox Regulation: Dithiol–Disulfide Equilibrium in Serine/ Threonine Phosphatase Calcineurin 306
Chapter 27. Glutathione Reductase from Bovine Brain 316
Chapter 28. Redox-Mediated Functional and Structural Changes in Insulin Receptor Kinase 323
Chapter 29. Redox Regulation of Protein Tyrosine Phosphatases by Hydrogen Peroxide: Detecting Sulfenic Acid Intermediates and Examining Reversible Inactivation 332
Chapter 30. Protein Cross-Linking by Self-Assisted Intermolecular Disulfide Bond Formation 341
Chapter 31. Sulfhydryl Oxidases as Factors for Mitochondrial Biogenesis 349
Chapter 32. Activation of Iron Regulatory Protein-1 by Oxidative Stress 359
Chapter 33. Mouse Astrocyte Cultures Used to Study Antioxidant Property of Metallothionein Isoforms 372
Chapter 34. Model Peptide Substrates and Ligands in Analysis of Action of Mammalian Protein Disulfide-Isomerase 377
Chapter 35. Escherichia coli SoxR Protein: Sensor/Transducer of Oxidative Stress and Nitric Oxide 390
Author Index 400
Subject Index 428

Produkt-Details

• Erscheint lt. Verlag: 6.3.2002
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Zoologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-049696-2 / 0080496962
• ISBN-13: 978-0-08-049696-2 / 9780080496962