Protein Sensors of Reactive Oxygen Species, Part A: Selenoproteins and Thioredoxin (eBook)

Lester Packer, Helmut Sies (Herausgeber)

eBook Download: EPUB

2002 | 1. Auflage
511 Seiten
Elsevier Science (Verlag)
978-0-08-049695-5 (ISBN)

Infos

This volume of Methods in Enzymology is concerned with the rapidly developing field of selenoprotein synthesis and its related molecular genetics. Progressive information on the topics of proteins as redox sensors, selenoproteins, and the thioredoxin system is studied using methods such as bioinformatics, DNA chip technology, cell biology, molecular genetics, and enzymology. The information on novel selenoproteins identified from genomic sequence data, as well as current knowledge on glutathione peroxidases, selenoprotein P, iodothyronine deiodinases, and thioredoxin reductases, is presented in a method-based approach.

Inhalt

Front Cover 1
Protein Sensors and Reactive Oxygen Species 4
Copyright Page 5
Table of Contents 6
Contributors to Volume 347 10
Preface 16
Volume in Series 18
Section I: Selenoproteins 38
Chapter 1. Selenoprotein Biosynthesis: Purification and Assay of Components Involved in Selenocysteine Biosynthesis and Insertion in Escherichia coli 40
Chapter 2. Selenocysteine Insertion Sequence Element Characterization and Selenoprotein Expression 54
Chapter 3. Transfer RNAs That Insert Selenocysteine 61
Chapter 4. Purification and Analysis of Selenocysteine Insertion Sequence-Binding Protein 2 77
Chapter 5. Nonsense-Mediated Decay: Assaying for Effects on Selenoprotein mRNAs 86
Chapter 6. Novel Selenoproteins Identified from Genomic Sequence Data 94
Chapter 7. Semisynthesis of Proteins Containing Selenocysteine 107
Chapter 8. Mammalian Selenoprotein Gene Signature: Identification and Functional Analysis of Selenoprotein Genes Using Bioinformatics Methods 121
Chapter 9. Estimation of Individual Types of Glutathione Peroxidases 138
Chapter 10. High-Throughput 96-Well Microplate Assays for Determining Specific Activities of Glutathione Peroxidase and Thioredoxin Reductase 150
Chapter 11. Selenoprotein P 158
Chapter 12. Iodothyronine Deiodinases 162
Chapter 13. Expression and Regulation of Thioredoxin Reductases and Other Selenoproteins in Bone 205
Chapter 14. Selenoprotein W 216
Chapter 15. Genetic and Functional Analysis of Mammalian Sep15 Selenoprotein 224
Chapter 16. Selenocysteine Lyase from Mouse Liver 235
Chapter 17. Selenocysteine Methyltransferase 240
Chapter 18. Phospholipid–Hydroperoxide Glutathione Peroxidase in Sperm 245
Chapter 19. In Vivo Antioxidant Role of Glutathione Peroxidase: Evidence from Knockout Mice 250
Chapter 20. Recombinant Expression of Mammalian Selenocysteine- Containing Thioredoxin Reductase and Other Selenoproteins in Escherichia coli 263
Chapter 21. Mammalian Thioredoxin Reductases as Hydroperoxide Reductases 273
Chapter 22. Tryparedoxin and Tryparedoxin Peroxidase 281
Chapter 23. Trypanothione and Tryparedoxin in Ribonucleotide Reduction 296
Chapter 24. Selenium- and Vitamin E-Dependent Gene Expression in Rats: Analysis of Differentially Expressed mRNAs 304
Section II: Thioredoxin 314
Chapter 25. Overview 316
Chapter 26. Thioredoxin and Glutaredoxin Isoforms 323
Chapter 27. Mammalian Thioredoxin Reductases 334
Chapter 28. Mitochondrial Thioredoxin Reductase and Thiol Status 344
Chapter 29. Protein Electrophoretic Mobility Shift Assay to Monitor Redox State of Thioredoxin in Cells 354
Chapter 30. Recycling of Vitamin C by Mammalian Thioredoxin Reductase 364
Chapter 31. Thioredoxin Cytokine Action 369
Chapter 32. Identification of Thioredoxin-Linked Proteins by Fluorescence Labeling Combined with Isoelectric Focusing/Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis 376
Chapter 33. Thioredoxin and Mechanism of Inflammatory Response 386
Chapter 34. Redox State of Cytoplasmic Thioredoxin 397
Chapter 35. Thioredoxin, Thioredoxin Reductase, and Thioredoxin Peroxidase of Malaria Parasite Plasmodium falciparum 407
Chapter 36. Human Placenta Thioredoxin Reductase: Preparation and Inhibitor Studies 419
Chapter 37. Classification of Plant Thioredoxins by Sequence Similarity and Intron Position 431
Chapter 38. Ferredoxin-Dependent Thioredoxin Reductase: A Unique Iron–Sulfur Protein 440
Chapter 39. Plant Thioredoxin Gene Expression: Control by Light, Circadian Clock, and Heavy Metals 449
Chapter 40. Thioredoxin Genes in Lens: Regulation by Oxidative Stress 458
Chapter 41. Thioredoxin Overexpression in Transgenic Mice 473
Chapter 42. Multiplex Reverse Transcription-Polymerase Chain Reaction for Determining Transcriptional Regulation of Thioredoxin and Glutaredoxin Pathways 478
Chapter 43. Redox Regulation of Cell Signaling by Thioredoxin Reductases 488
Author Index 500
Subject Index 534

Produkt-Details

• Erscheint lt. Verlag: 12.3.2002
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-049695-4 / 0080496954
• ISBN-13: 978-0-08-049695-5 / 9780080496955