Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater

T. Cromeans (Autor)

Buch | Softcover

78 Seiten

2004
IWA Publishing (Verlag)
978-1-84339-722-9 (ISBN)

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Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.

Reihe/Serie	WERF Research Report Series
Verlagsort	London
Sprache	englisch
Maße	156 x 234 mm
Themenwelt	Technik ► Umwelttechnik / Biotechnologie
ISBN-10	1-84339-722-6 / 1843397226
ISBN-13	978-1-84339-722-9 / 9781843397229
Zustand	Neuware