Genotype Phenotype Coupling
Springer-Verlag New York Inc.
978-1-0716-3281-9 (ISBN)
Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.
Quantitative Determination of Staphylococcus aureus Using Aptamer-Based Recognition and DNA Amplification Machinery.- Construction of Synthetic VHH Libraries in Ribosome Display Format.- Isolation of Adhirons Specific for Plant Protoplast Membrane Biomarkers is Simplified by Phagemid Design.- Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage Display by Golden Gate Cloning.- Identification of New Antibodies Targeting Tumor Cell Surface Antigens by Phage Display.- Phage Display of Bovine Ultralong CDRH3.- Bacterial Cell Display for Selection of Affibody Molecules.- Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display.- Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Library.- A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries.- Single-Cell B-Cell Sequencing to Generate Natively-Paired scFab Yeast Surface Display Libraries.- One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries.- Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display.- Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening.- Tyrosine Phosphorylation Screening on the Yeast Surface by Magnetic Bead Selection and FACS.- Bulk Reformatting of Antibody Fragments Displayed on the Surface of Yeast Cells to Final IgG Format for Mammalian Production.- Antibody Secreting Cell Isolation from Different Species for Microfluidic Antibody Hit Discovery.- Efficient Microfluidics Downstream Processes for Rapid Antibody Hit Confirmation.- Cell Line Development Using Targeted Gene Integration into MAR-Rich Landing Pads for Stable Expression of Transgenes.- Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression.- Antibody Display Technology (ADbody) to Present Challenging and Unstable Target Proteins on Antibodies.- SUMO: In Silico Sequence Assessment Using Multiple Optimization Parameters.- Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum.
| Erscheinungsdatum | 07.07.2024 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 65 Illustrations, color; 10 Illustrations, black and white; XV, 408 p. 75 illus., 65 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Technik ► Umwelttechnik / Biotechnologie | |
| Schlagworte | Alternative scaffolds • antibodies • Antibody engineering and discovery • Natively paired antibody libraries • Single cell technologies • therapeutics |
| ISBN-10 | 1-0716-3281-7 / 1071632817 |
| ISBN-13 | 978-1-0716-3281-9 / 9781071632819 |
| Zustand | Neuware |
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