Protocols for Oligonucleotide Conjugates
Humana Press Inc. (Verlag)
978-0-89603-252-1 (ISBN)
D ring the past ten ears or so, there has been an increasing n mber of p blications describing ario s aspects of oligon cleotide chemistr and the application of oligon cleotides in basic, as ell as applied science. More recentl , ario s oligon cleotide conj gates nthesi ed that are c rrentl being st died for their ha e been s potential diagnostic and therape tic applications. With the introd- tion of ne oligon cleotide conj gates, anal tical methods for the anal sis of these oligon cleotides are also being de eloped and op- mi ed. The present ol me, Protocols for Oligon cleotide Con- gates: S nthesis and Anal tical Techniq es, is designed to bring readers p-to-date on the protocols for the s nthesis of ario s oli- gates and on all the important techniq es for the n cleotide conj nthetic oligon cleotides and their analogs. The s- anal sis of s thetic chemistrof man of these oligon cleotides and their ario s analogs has been co ered in a companion ol me entitledProtocols for Oligon cleotides and Analogs: S nthesis and Properties. The first chapter ofProtocols for Oligon cleotide Conj gates- scribes the protecting gro ps in oligon cleotide s nthesis.
Protecting Groups in Oligonucleotide Synthesis.- Incorporation of Modified Bases into Oligonucleotides.- Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups.- Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups.- Postsynthesis Functionalization of Oligonucleotides.- Oligonucleotide-Enzyme Conjugates.- Oligonucleotides Containing Degenerate Bases.- Synthesis of [15N]-Labeled DNA Fragments.- Analysis and Purification of Synthetic Oligonucleotides by High-Performance Liquid Chromatography.- Sequence Analysis of Oligodeoxyribonucleotides.- Gel-Capillary Electrophoresis Analysis of Oligonucleotides.- Nuclear Magnetic Resonance Studies of Oligonucleotides.- Mass Spectrometry of Nucleotides and Oligonucleotides.- Extracting Thermodynamic Data From Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions.
...an extremely useful and well-produced volume...a very well-qualified and prestigious group of authors...produce an up-to-date review of current topics in this important and growing field. - Trends in Biotechnology
| Erscheint lt. Verlag | 30.11.1993 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 26 |
| Zusatzinfo | XIV, 377 p. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Naturwissenschaften ► Chemie ► Organische Chemie | |
| Technik ► Umwelttechnik / Biotechnologie | |
| ISBN-10 | 0-89603-252-3 / 0896032523 |
| ISBN-13 | 978-0-89603-252-1 / 9780896032521 |
| Zustand | Neuware |
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