Yeast Metabolic Engineering
Springer-Verlag New York Inc.
978-1-0716-2398-5 (ISBN)
Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae.- High Efficiency Plasmid DNA Transformation in Yeast.- CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae.- Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening.- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology.- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.- Competent Cell Preparation and Transformation of Pichia pastoris.- CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome.- Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris.- Engineering of Promoters for Gene Expression in Pichia pastoris.- Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory.- Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica.- A Universal Gene Expression System for Novel Yeast Species.- Dynamic Feeding of Pichia pastoris.- Automated Evolutionary Engineering of Yeasts.- Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN.
Erscheinungsdatum | 08.07.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2513 |
Zusatzinfo | 32 Illustrations, color; 19 Illustrations, black and white; XI, 295 p. 51 illus., 32 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
Naturwissenschaften ► Chemie ► Analytische Chemie | |
Technik ► Maschinenbau | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | chassis designs • CRISPR-Cas • Lipidomics • Microscopy • Pathway Activity Profiling (PAPi) |
ISBN-10 | 1-0716-2398-2 / 1071623982 |
ISBN-13 | 978-1-0716-2398-5 / 9781071623985 |
Zustand | Neuware |
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