Microtubules
Springer-Verlag New York Inc.
978-1-0716-1982-7 (ISBN)
Authoritative and cutting-edge, Microtubules: Methods and Protocols aims to provide useful information for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols.
Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification.- Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule Associated Intracellular Function.- Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA.- Electro-Modulation of Tubulin Properties and Function.- In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference Reflection Microscopy.- Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microscopy.- Design of Mechanical and Electrical Properties for Multi-Directional Control of Microtubules.- Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay.- Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices.- Assembling Microtubule-Based Active Matter.- Spontaneous Alignment of Microtubules via Tubulin Polymerization in a Narrow Space under a Temperature Gradient.- Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli.- Spontaneously Beating Biomimetic Structures.- Construction of Molecular Robots from Microtubules for Programmable Swarming.- Fabrication of Artificial Muscle from Microtubules, Kinesins and DNA Origami Nanostructures.- Encapsulation of Nanomaterials Inside Microtubules by Using a Tau-derived Peptide.- Investigating Tubulin-Drug Interaction Using Fluorescence Spectroscopy.- Visualization and Quantification of Microtubule Self-repair.- Cargo Transport by Microtubule-Associated Motor Protein Along Mechanically Deformed Microtubules.- Mechanical Deformation of Microtubules on a Two-Dimensional Elastic Medium.- Real-Time Imaging of Single γTuRC-Mediated Microtubule Nucleation Events In Vitro by TIRF Microscopy.- Microtubule Preparation for Investigation with High-Speed Atomic Force Microscopy.- Crystallization Systems for the High-Resolution Structural Analysis of Tubulin-Ligand Complexes.- Cryo-EM Visualization of Neuronal Particles Inside Microtubules.- Reconstituting the Interaction Between Purified Nuclei and Microtubule Network.- Photocontrolling Microtubule Dynamics with Photoswitchable Chemical Reagents.- Monitoring the Disruptive Effects of Tubulin Binding Agents on Cellular Microtubules.- Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules.- Controlling Cell Shape and Microtubule Organization by Extracellular Matrix Micropatterning.
| Erscheinungsdatum | 03.05.2022 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2430 |
| Zusatzinfo | 110 Illustrations, color; 21 Illustrations, black and white; XIV, 486 p. 131 illus., 110 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie |
| Technik ► Medizintechnik | |
| Schlagworte | cytoskeletons • microtubule-based materials • microtubule-binding molecules • motor proteins |
| ISBN-10 | 1-0716-1982-9 / 1071619829 |
| ISBN-13 | 978-1-0716-1982-7 / 9781071619827 |
| Zustand | Neuware |
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