Metabolic Pathway Engineering

Michael E. Himmel, Yannick J. Bomble (Herausgeber)

Buch | Softcover

236 Seiten

2021 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0197-6 (ISBN)

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This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost.
Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.

Overview and Future Directions.- Genetics of Unstudied Thermophiles for Industry.- Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum.- Methods for Metabolic Engineering of a Filamentous Trichoderma reesei.- Methods for Algal Protein Isolation and Proteome Analysis.- An Improved Leaf Protoplast System for Highly Efficient Transient Expression in Switchgrass (Panicum virgatum L.).- Characterizing Intracellular Proteomes for Microbes: An Experimental Approach Using Label-Free Protein Quantitation.- Bacterial Differential Expression Analysis Methods.- Measuring Biomass-Derived Products in Biological Conversion and Metabolic Process.- Crystallography of Metabolic Enzymes.- Measuring Metabolic Enzyme Performance.- Gene Editing Technologies for Biofuel Production in Thermophilic Microbes.- Software and Methods for Computational Flux Balance Analysis.- Dynamic Flux Analysis: An Experimental Approach of Fluxomics.- Network Modeling of Complex Data Sets.- Connecting Microbial Genotype with Phenotype in the Omics Era.

Erscheinungsdatum	12.08.2021
Reihe/Serie	Methods in Molecular Biology ; 2096
Zusatzinfo	48 Illustrations, color; 8 Illustrations, black and white; XI, 236 p. 56 illus., 48 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Technik ► Medizintechnik
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Biochemicals • Computational methodologies • Enzyme performance • flux analysis • fuels • Industrial Processes
ISBN-10	1-0716-0197-0 / 1071601970
ISBN-13	978-1-0716-0197-6 / 9781071601976
Zustand	Neuware