Corneal Regeneration

Methods and Protocols

Buch | Softcover

250 Seiten

2021 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0601-8 (ISBN)

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This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Isolation and Culture of Corneal Stromal Stem Cells.- In vitro Expansion of Corneal Endothelial Cells for Transplantation.- Primary Culture of Cornea-Limbal Epithelial Cells In Vitro.- Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant.- A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture.- Gene Editing for Corneal Stromal Regeneration.- Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery.-The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea.- Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding.- Preparation of Dried Amniotic Membrane for Corneal Repair.- Fabrication of Corneal Extracellular Matrix Derived Hydrogels.- Synthesis and Application of Collagens for Assembling a Corneal Implant.- Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts.- Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration.- The use of Animal Models to Assess Engineered Corneal Tissue.- X-ray Diffraction Imaging of Corneal Ultrastructure.

Erscheinungsdatum	28.06.2021
Reihe/Serie	Methods in Molecular Biology ; 2145
Zusatzinfo	43 Illustrations, color; 23 Illustrations, black and white; XII, 250 p. 66 illus., 43 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Augenheilkunde
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Biomedizin
	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Technik ► Medizintechnik
	Technik ► Umwelttechnik / Biotechnologie
ISBN-10	1-0716-0601-8 / 1071606018
ISBN-13	978-1-0716-0601-8 / 9781071606018
Zustand	Neuware